Abstract：
Antimicrobial peptides (AMPs) are considered a novel approach to stimulate fish antiviral mechanisms for defense against a broad range of viral infections by enhancing immunomodulatory activities. Octominin is an AMP derived from the defense proteins of Octopus minor. In this study, preliminary screening of octominin against viral hemorrhagic septicemia virus (VHSV), infectious hematopoietic necrosis virus (IHNV), and infectious pancreatic necrosis virus (IPNV) was carried out. Moreover, immune responses upon octominin treatment and IHNV challenge were investigated using fathead minnow (FHM) cells. The CC50s of octominin for FHM and Chinook salmon embryo-214 (CHSE-214) cells were 2146.2 and 1865.2 μg/mL, respectively. With octominin treatment, EC50 resulted in 732.8, 435.1, and 925.9 μg/mL for VHSV, IHNV, and IPNV, respectively. The selectivity indices were 2.9, 4.9, and 2.0, respectively. The transcriptional analysis results demonstrated the induced transcription factors (Irf3; 143-fold, Irf7; 105-fold, and NF-κB; 8-fold), stress response gene (HspB8; 2-fold), and apoptosis functional gene (p53; 3-fold) in octominin treated (500 μg/mL) FHM cells for 48 h. Moreover, IHNV viral copy number was slightly decreased with the octominin treatment (500 μg/mL) in FHM cells. Overall results suggest that octominin could be a potential antiviral agent, although further studies are necessary to understand its mode of action and the mechanism of its antiviral activity.
摘要：
抗菌肽（AMP）被认为是一种通过增强免疫调节活性来刺激鱼类抗病毒机制以防御各种病毒感染的新方法。Octominin是源自小章鱼防御蛋白的AMP。在这项研究中,针对病毒性出血性败血病病毒（VHSV）的octominin的初步筛查，传染性造血系统坏死病毒(IHNV),并进行了传染性胰腺坏死病毒（IPNV）。此外，使用黑头小牛（FHM）细胞研究了octominin治疗和IHNV攻击后的免疫反应。FHM和奇努克鲑鱼胚胎214（CHSE-214）细胞的octominin的CC50分别为2146.2和1865.2μg/mL，分别。用octominin治疗，VHSV的EC50分别为732.8、435.1和925.9μg/mL，IHNV,和IPNV，分别。选择指数为2.9、4.9和2.0。转录分析结果表明诱导的转录因子(Irf3;143倍，Irf7；105倍，和NF-κB；8倍），应激反应基因（HspB8；2倍），和凋亡功能基因（p53；3倍）在octominin处理（500μg/mL）FHM细胞48小时。此外，在FHM细胞中,用octominin处理(500μg/mL),IHNV病毒拷贝数略微降低。总体结果表明，octominin可能是一种潜在的抗病毒药物，虽然进一步的研究是必要的，以了解其作用方式和其抗病毒活性的机制。
