Abstract：
Legionnaires\' disease is a severe form of pneumonia caused by the inhalation or aspiration of water droplets contaminated with Legionella pneumophila and other Legionella species. These bacteria are commonly found in natural habitats and man-made water systems. Legionnaires\' disease is a significant public health problem, especially in healthcare settings where patients may be exposed to contaminated environmental sources. Nosocomial outbreaks have been reported worldwide, leading to high morbidity and mortality rates, and increased healthcare costs. This study aimed to compare, the clonal relationship of clinical L. pneumophila strains from two different hospitals with L. pneumophila strains isolated from the water supply.
In the period from 2019 to 2021, clinical and environmental strains involved in three cases of legionellosis were compared by means of pulsed field gel electrophoresis and sequence based typing techniques.
Our findings highlight the persistence of clonally distinct strains within each hospital examined. Furthermore, the L. pneumophila strains detected from hospital environmental sources were related to the clinical strains isolated, demonstrating the nosocomial origin of these cases.
Therefore, it is important to implement more accurate surveillance systems both for epidemiological studies and to check the effectiveness of remediation procedures. (www.actabiomedica.it).
In the period from 2019 to 2021, clinical and environmental strains involved in three cases of legionellosis were compared by means of pulsed field gel electrophoresis and sequence based typing techniques.
Our findings highlight the persistence of clonally distinct strains within each hospital examined. Furthermore, the L. pneumophila strains detected from hospital environmental sources were related to the clinical strains isolated, demonstrating the nosocomial origin of these cases.
Therefore, it is important to implement more accurate surveillance systems both for epidemiological studies and to check the effectiveness of remediation procedures. (www.actabiomedica.it).
摘要：
目的：军团菌病是一种严重的肺炎，由吸入或吸入被嗜肺军团菌和其他军团菌污染的水滴引起。这些细菌通常在自然栖息地和人造水系统中发现。军团病是一个重大的公共卫生问题，特别是在医疗环境中，病人可能会暴露在污染的环境来源。全世界都有医院爆发的报道，导致高发病率和死亡率，增加医疗费用。本研究旨在比较，来自两家不同医院的临床嗜肺乳杆菌菌株与从供水中分离的嗜肺乳杆菌菌株的克隆关系。
方法：在2019年至2021年期间，通过脉冲场凝胶电泳和基于序列的分型技术，比较了3例军团菌病的临床和环境菌株。
结果：我们的发现强调了在所检查的每个医院中克隆不同菌株的持续存在。此外，从医院环境来源检测到的嗜肺乳杆菌菌株与分离的临床菌株有关，证明这些病例的医院起源。
结论：因此，对于流行病学研究和检查补救措施的有效性，实施更准确的监测系统非常重要。(www.actabiomedica.it).
方法：在2019年至2021年期间，通过脉冲场凝胶电泳和基于序列的分型技术，比较了3例军团菌病的临床和环境菌株。
结果：我们的发现强调了在所检查的每个医院中克隆不同菌株的持续存在。此外，从医院环境来源检测到的嗜肺乳杆菌菌株与分离的临床菌株有关，证明这些病例的医院起源。
结论：因此，对于流行病学研究和检查补救措施的有效性，实施更准确的监测系统非常重要。(www.actabiomedica.it).
