Abstract：
OBJECTIVE: Perinatal hypoxic-ischemic encephalopathy (HIE) is a condition that can lead to long-term cognitive, motor, and behavioral impairments in newborns. Although brain hypothermia therapy is currently the standard treatment for HIE, it does not provide complete neuroprotection. As a result, there is a need to explore additional therapies to enhance treatment outcomes. This study aims to investigate the potential role of Ginkgolide B (GB) in promoting neuroplasticity and facilitating spontaneous recovery after HIE.
METHODS: In this study, we employed a neonatal rat model of HIE to investigate the effects of GB on spontaneous recovery. GB treatment was initiated 24 h after hypoxia and administered continuously for a duration of 14 days. We evaluated several outcome measures after the treatment period, including spontaneous behavioral recovery and brain repair. Additionally, we quantified the levels of netrin-1 in both plasma and the peri-ischemic zone after the occurrence of HIE.
RESULTS: We found that GB treatment significantly facilitated spontaneous behavioral recovery in the HIE pups. Furthermore, cognitive function was restored, and brain tissue repair had a noticeable acceleration. We observed increased cell proliferation in the subventricular, stratum, and subgranular zones. Of particular interest, we observed elevated levels of netrin-1 in both plasma and the ischemic penumbra following GB treatment.
CONCLUSIONS: Our findings suggest that GB promotes neuroplasticity and enhances spontaneous recovery in newborns affected by HIE. The observed upregulation of netrin-1 may be crucial in mediating these effects. These results highlight the promising potential of GB as a post-HIE therapy, particularly in enhancing spontaneous recovery and improving long-term outcomes.
METHODS: In this study, we employed a neonatal rat model of HIE to investigate the effects of GB on spontaneous recovery. GB treatment was initiated 24 h after hypoxia and administered continuously for a duration of 14 days. We evaluated several outcome measures after the treatment period, including spontaneous behavioral recovery and brain repair. Additionally, we quantified the levels of netrin-1 in both plasma and the peri-ischemic zone after the occurrence of HIE.
RESULTS: We found that GB treatment significantly facilitated spontaneous behavioral recovery in the HIE pups. Furthermore, cognitive function was restored, and brain tissue repair had a noticeable acceleration. We observed increased cell proliferation in the subventricular, stratum, and subgranular zones. Of particular interest, we observed elevated levels of netrin-1 in both plasma and the ischemic penumbra following GB treatment.
CONCLUSIONS: Our findings suggest that GB promotes neuroplasticity and enhances spontaneous recovery in newborns affected by HIE. The observed upregulation of netrin-1 may be crucial in mediating these effects. These results highlight the promising potential of GB as a post-HIE therapy, particularly in enhancing spontaneous recovery and improving long-term outcomes.
摘要：
目的：围产期缺氧缺血性脑病（HIE）是一种可导致长期认知，电机,和新生儿的行为障碍。尽管脑低温疗法目前是HIE的标准治疗方法，它不能提供完整的神经保护。因此,有必要探索额外的治疗方法以提高治疗效果.本研究旨在探讨银杏内酯B（GB）在促进HIE后神经可塑性和促进自发恢复中的潜在作用。
方法：在本研究中，我们采用新生大鼠HIE模型研究GB对自然恢复的影响。在缺氧后24小时开始GB治疗并连续给药14天。我们评估了治疗期后的几个结果指标，包括自发的行为恢复和大脑修复。此外,我们量化了HIE发生后血浆和缺血周围区的netrin-1水平.
结果：我们发现GB治疗显著促进了HIE幼崽的自发行为恢复。此外，认知功能恢复,脑组织修复有明显的加速.我们观察到脑室下细胞增殖增加，地层,和亚粒区。特别感兴趣的是，我们观察到GB治疗后血浆和缺血半影区的netrin-1水平升高.
结论：我们的研究结果表明，GB促进受HIE影响的新生儿的神经可塑性和自发恢复。观察到的netrin-1的上调可能对介导这些作用至关重要。这些结果凸显了GB作为HIE后治疗的潜力，特别是在促进自发恢复和改善长期结果方面。
方法：在本研究中，我们采用新生大鼠HIE模型研究GB对自然恢复的影响。在缺氧后24小时开始GB治疗并连续给药14天。我们评估了治疗期后的几个结果指标，包括自发的行为恢复和大脑修复。此外,我们量化了HIE发生后血浆和缺血周围区的netrin-1水平.
结果：我们发现GB治疗显著促进了HIE幼崽的自发行为恢复。此外，认知功能恢复,脑组织修复有明显的加速.我们观察到脑室下细胞增殖增加，地层,和亚粒区。特别感兴趣的是，我们观察到GB治疗后血浆和缺血半影区的netrin-1水平升高.
结论：我们的研究结果表明，GB促进受HIE影响的新生儿的神经可塑性和自发恢复。观察到的netrin-1的上调可能对介导这些作用至关重要。这些结果凸显了GB作为HIE后治疗的潜力，特别是在促进自发恢复和改善长期结果方面。
