Abstract：
Vulvovaginal candidiasis (VVC) is a fungal infection that is a global issue of women\'s health due to its association with morbidity, infertility, and economic costs. This study aimed to compare the vitamin D3 levels between women with VVC to healthy controls and determine the species distribution and susceptibility pattern of isolates. Species identification was performed using sequencing of the ITS-rDNA regions and amplification of the HWP1 gene. Antifungal susceptibility testing was determined by the disk diffusion method. Moreover, serum vitamin D3 levels were measured using a commercial ELISA (enzyme-linked immunosorbent assay) kit. Our results indicated that vitamin D3 level in women with VVC was lower than those of healthy women (p-value < .001). Candida albicans complex (62.8 percent) was the most common species, and most species were susceptible to fluconazole, itraconazole, ketoconazole, and nystatin. In conclusion, our study revealed a potential link between vitamin D3 deficiency and VVC in women. Although our findings showed significantly lower vitamin D3 levels in women with VVC, further research is needed to establish a definitive causative relationship between vitamin D3 deficiency and VVC. Nonetheless, our study highlights the potential importance of maintaining adequate levels of vitamin D3 and the need for further exploration in this area.
摘要：
外阴阴道念珠菌病（VVC）是一种真菌感染，是一个全球性的妇女健康问题，由于其与发病率相关，不孕症,和经济成本。这项研究旨在比较VVC女性与健康对照之间的维生素D3水平，并确定分离株的物种分布和易感性模式。使用ITS-rDNA区域的测序和HWP1基因的扩增进行物种鉴定。抗真菌药敏试验通过圆盘扩散法测定。此外，采用市售ELISA(酶联免疫吸附测定)试剂盒测定血清维生素D3水平.我们的结果表明，VVC女性的维生素D3水平低于健康女性（p值<.001）。白色念珠菌复合体（62.8％）是最常见的物种，大多数物种对氟康唑敏感，伊曲康唑,酮康唑,还有制霉菌素.总之,我们的研究揭示了女性维生素D3缺乏与VVC之间的潜在联系.尽管我们的研究结果显示，VVC女性的维生素D3水平明显降低，需要进一步的研究来确定维生素D3缺乏与VVC之间的明确因果关系.尽管如此，我们的研究强调了维持维生素D3充足水平的潜在重要性,以及在这方面需要进一步探索.
