PDF(Pubmed)
Abstract：
Hemorrhagic fever with renal syndrome (HFRS) is a zoonotic disease transmitted by several rodent species. We obtained clinical data of HFRS patients from the medical records of the People\'s Hospital of Xiangyun County in Dali Prefecture from July 2019 to August 2021. We collected epidemiological data of HFRS patients through interviews and investigated host animals using the night clip or night cage method. We systematically performed epidemiological analyses of patients and host animals. The differences in the presence of rodent activity at home (χ2 = 8.75, p = 0.031 < 0.05), of rodent-proof equipment in the food (χ2 = 9.19, p = 0.025 < 0.05), and of rodents or rodent excrement in the workplace (χ2 = 10.35, p = 0.014 < 0.05) were statistically different in the four clinical types, including mild, medium, severe, and critical HFRS-associated diseases. Furthermore, we conducted molecular detection of orthohantavirus in host animals. The total orthohantavirus infection rate of rodents was 2.72% (9/331); the specific infection rate of specific animal species was 6.10% (5/82) for the Apodemus chevrieri, 100% (1/1) for the Rattus nitidus, 3.77% (2/53) for the Rattus norvegicus, and 12.50% (1/8) for the Crocidura dracula. In this study, a total of 21 strains of orthohantavirus were detected in patients and rodents. The 12 orthohantavirus strains from patients showed a closer relationship with Seoul orthohantavirus (SEOOV) L0199, DLR2, and GZRn60 strains; the six orthohantavirus strains from Rattus norvegicus and Apodemus chevrieri were closely related to SEOOV GZRn60 strain. One strain (XYRn163) from Rattus norvegicus and one strain (XYR.nitidus97) from Rattus nitidus were closely related to SEOOV DLR2 strain; the orthohantavirus strain from Crocidura dracula was closely related to the Luxi orthohantavirus (LUXV) LX309 strain. In conclusion, patients with HFRS in Xuangyun County of Dali Prefecture are predominantly affected by SEOOV, with multiple genotypes of orthohantavirus in host animals, and, most importantly, these orthohantavirus strains constantly demonstrated zoonotic risk in humans.
摘要：
肾综合征出血热（HFRS）是由几种啮齿动物传播的人畜共患疾病。我们从大理州祥云县人民医院2019年7月至2021年8月的病历中获取HFRS患者的临床资料。我们通过访谈收集了HFRS患者的流行病学数据，并使用夜夹或夜笼方法对宿主动物进行了调查。我们系统地对患者和宿主动物进行了流行病学分析。在家中存在啮齿动物活动的差异（χ2=8.75，p=0.031<0.05），食品中的防鼠设备（χ2=9.19，p=0.025<0.05），和工作场所的啮齿动物或啮齿动物粪便（χ2=10.35，p=0.014<0.05）在四种临床类型中具有统计学差异，包括轻度,中等,严重,和严重的HFRS相关疾病。此外，我们在宿主动物中进行了正坦病毒的分子检测。啮齿类动物的总正体病毒感染率为2.72%(9/331);特定动物物种的特定感染率为6.10%(5/82),100%(1/1)的白鼠，褐家鼠3.77%(2/53)，和12.50％（1/8）的蛇。在这项研究中,在患者和啮齿动物中共检测到21株正坦病毒。来自患者的12株正畸病毒株与首尔正畸病毒（SEOOV）L0199，DLR2和GZRn60毒株有更密切的关系；来自褐家鼠和变形杆菌的6株正畸病毒株与SEOOVGZRn60毒株密切相关。一只来自褐家鼠的菌株（XYRn163）和一只菌株（XYR。来自白鼠的nitidus97）与SEOOVDLR2毒株密切相关；来自Crociduradrad的正坦病毒毒株与鲁西正坦病毒（LUXV）LX309毒株密切相关。总之,大理州宣云县的HFRS患者主要受到SEOOV的影响，在宿主动物中具有多种基因型的正坦病毒，and,最重要的是,这些正畸病毒株在人类中不断表现出人畜共患风险。
