PDF(Pubmed)
Abstract：
The asymptomatic nature, high rate of disease recurrence, and resistance to platinum-based chemotherapy highlight the need to identify and characterize novel target molecules for ovarian cancer. Fibroblast growth factor 8 (FGF8) aids in the development and metastasis of ovarian cancer; however, its definite role is not clear. We employed ELISA and IHC to examine the expression of FGF8 in the saliva and tissue samples of epithelial ovarian cancer (EOC) patients and controls. Furthermore, various cell assays were conducted to determine how FGF8 silencing influences ovarian cancer cell survival, adhesion, migration, and invasion to learn more about the functions of FGF8. In saliva samples, from controls through low-grade to high-grade EOC, a stepped overexpression of FGF8 was observed. Similar expression trends were seen in tissue samples, both at protein and mRNA levels. FGF8 gene silencing in SKOV3 cells adversely affected various cell properties essential for cancer cell survival and metastasis. A substantial reduction was observed in the cell survival, cell adhesion to the extracellular matrix, migration, and adhesion properties of SKOV3 cells, suggesting that FGF8 plays a crucial role in the development of EOC. Conclusively, this study suggests a pro-metastatic function of FGF8 in EOC.
摘要：
无症状性质，疾病复发率高,和对铂类化疗的耐药性突出了鉴定和表征卵巢癌新靶分子的必要性。成纤维细胞生长因子8（FGF8）有助于卵巢癌的发展和转移；然而，其确切作用尚不清楚。我们采用ELISA和IHC检查了上皮性卵巢癌（EOC）患者和对照组的唾液和组织样品中FGF8的表达。此外，进行各种细胞试验以确定FGF8沉默如何影响卵巢癌细胞存活，附着力,迁移,和入侵以了解有关FGF8功能的更多信息。在唾液样本中，从控制到低级到高级EOC，观察到FGF8的阶梯式过表达。在组织样本中观察到类似的表达趋势，在蛋白质和mRNA水平。SKOV3细胞中的FGF8基因沉默不利地影响癌细胞存活和转移所必需的各种细胞特性。观察到细胞存活率大幅降低，细胞与细胞外基质的粘附，迁移,和SKOV3细胞的粘附特性，表明FGF8在EOC的发展中起着至关重要的作用。最后，这项研究表明FGF8在EOC中具有促转移功能。
