PDF(Pubmed)
Abstract：
Climate change results in exceptional environmental conditions and drives the migration of pathogens to which local plants are not adapted. Biotic stress disrupts plants\' metabolism, fitness, and performance, ultimately impacting their productivity. It is therefore necessary to develop strategies for improving plant resistance by promoting stress responsiveness and resilience in an environmentally friendly and sustainable way. The aim of this study was to investigate whether priming tobacco plants with a formulation containing silicon-stabilised hybrid lipid nanoparticles functionalised with quercetin (referred to as GS3 phyto-courier) can protect against biotic stress triggered by Agrobacterium tumefaciens leaf infiltration. Tobacco leaves were primed via infiltration or spraying with the GS3 phyto-courier, as well as with a buffer (B) and free quercetin (Q) solution serving as controls prior to the biotic stress. Leaves were then sampled four days after bacterial infiltration for gene expression analysis and microscopy. The investigated genes increased in expression after stress, both in leaves treated with the phyto-courier and control solutions. A trend towards lower values was observed in the presence of the GS3 phyto-courier for genes encoding chitinases and pathogenesis-related proteins. Agroinfiltrated leaves sprayed with GS3 confirmed the significant lower expression of the pathogenesis-related gene PR-1a and showed higher expression of peroxidase and serine threonine kinase. Microscopy revealed swelling of the chloroplasts in the parenchyma of stressed leaves treated with B; however, GS3 preserved the chloroplasts\' mean area under stress. Furthermore, the UV spectrum of free Q solution and of quercetin freshly extracted from GS3 revealed a different spectral signature with higher values of maximum absorbance (Amax) of the flavonoid in the latter, suggesting that the silicon-stabilised hybrid lipid nanoparticles protect quercetin against oxidative degradation.
摘要：
气候变化导致特殊的环境条件，并驱动当地植物不适应的病原体的迁移。生物胁迫破坏植物的新陈代谢，健身,和性能，最终影响他们的生产力。因此，有必要通过以环境友好和可持续的方式促进胁迫响应性和恢复力来制定改善植物抗性的策略。这项研究的目的是研究是否用包含硅稳定的混合脂质纳米颗粒的配方引发烟草植物用槲皮素（称为GS3phyto-couser）功能化可以防止根癌农杆菌叶片浸润引发的生物胁迫。通过渗透或喷洒GS3phyto-courier来灌注烟草叶，以及在生物胁迫之前用缓冲液(B)和游离槲皮素(Q)溶液作为对照。然后在细菌浸润后四天对叶片取样用于基因表达分析和显微镜检查。研究的基因在应激后表达增加，在用植物快递和控制溶液处理的叶子中。在存在编码几丁质酶和发病机理相关蛋白的基因的GS3phyto信使的情况下，观察到了较低值的趋势。喷洒GS3的农杆菌浸润叶片证实了发病相关基因PR-1a的显着较低表达，并显示出过氧化物酶和丝氨酸苏氨酸激酶的较高表达。显微镜检查显示，用B处理的应激叶片薄壁组织中的叶绿体肿胀；然而，GS3保留了压力下的叶绿体平均面积。此外，游离Q溶液和新鲜从GS3中提取的槲皮素的紫外光谱显示出不同的光谱特征，后者中类黄酮的最大吸光度（Amax）值较高，表明硅稳定的混合脂质纳米颗粒保护槲皮素免受氧化降解。
