PDF(Pubmed)
Abstract：
Although dry eye disease (DED) is one of the most common ocular surface diseases worldwide, its pathogenesis is incompletely understood, and treatment options are limited. There is growing evidence that complex interactions between the ocular surface microbiome (OSM) and tear fluid constituents, potentially leading to inflammatory processes, are associated with ocular surface diseases such as DED. In this study, we aimed to find unique compositional and functional features of the OSM associated with human and microbial tear proteins in patients with DED. Applying whole-metagenome shotgun sequencing of forty lid and conjunctival swabs, we identified 229 taxa, with Actinobacteria and Proteobacteria being the most abundant phyla and Propionibacterium acnes the dominating species in the cohort. When DED patients were compared to controls, the species Corynebacterium tuberculostearicum was more abundant in conjunctival samples, whereas the family Propionibacteriaceae was more abundant in lid samples. Functional analysis showed that genes of L-lysine biosynthesis, tetrapyrrole biosynthesis, 5-aminoimidazole ribonucleotide biosynthesis, and the super pathway of L-threonine biosynthesis were enriched in conjunctival samples of controls. The relative abundances of Acinetobacter johnsonii correlated with seven human tear proteins, including mucin-16. The three most abundant microbial tear proteins were the chaperone protein DnaK, the arsenical resistance protein ArsH, and helicase. Compositional and functional features of the OSM and the tear proteome are altered in patients with DED. Ultimately, this may help to design novel interventional therapeutics to target DED.
摘要：
尽管干眼病（DED）是全球最常见的眼表疾病之一，其发病机制尚未完全了解，和治疗选择是有限的。越来越多的证据表明，眼表微生物组（OSM）和泪液成分之间的复杂相互作用，可能导致炎症过程，与眼表疾病如DED有关。在这项研究中,我们旨在发现DED患者中与人类和微生物泪液蛋白相关的OSM的独特组成和功能特征。应用40个眼睑和结膜拭子的全宏基因组shot弹枪测序，我们确定了229个分类单元，放线菌和变形菌是最丰富的门，痤疮丙酸杆菌是队列中的主要物种。当DED患者与对照组比较时，结膜样品中结核杆菌的种类更丰富，而丙酸杆菌科在盖子样本中更为丰富。功能分析显示L-赖氨酸生物合成基因,四吡咯生物合成，5-氨基咪唑核糖核苷酸生物合成,在对照的结膜样品中富集了L-苏氨酸生物合成的超途径。约翰逊不动杆菌的相对丰度与7种人类泪液蛋白相关，包括黏蛋白-16.三种最丰富的微生物泪液蛋白是伴侣蛋白DnaK，砷抗性蛋白ArsH,和解旋酶。DED患者的OSM和泪液蛋白质组的组成和功能特征发生了改变。最终,这可能有助于设计针对DED的新型介入疗法。
