PDF(Pubmed)
Abstract：
In the pursuit of identifying the underlying pathways of ocular diseases, the use of cell lines such as (retinal ganglion cell-5) RGC-5 and 661W became a valuable tool, including pathologies like retinal degeneration and glaucoma. In 2001, the establishment of the RGC-5 cell line marked a significant breakthrough in glaucoma research. Over time, however, concerns arose about the true nature of RGC-5 cells, with conflicting findings in the literature regarding their identity as retinal ganglion cells or photoreceptor-like cells. This study aimed to address the controversy surrounding the RGC-5 cell line\'s origin and properties by comparing it with the 661W cell line, a known cone photoreceptor model. Both cell lines were differentiated according to two prior published redifferentiation protocols under the same conditions using 500 nM of trichostatin A (TSA) and investigated for their morphological and neuronal marker properties. The results demonstrated that both cell lines are murine, and they exhibited distinct morphological and neuronal marker properties. Notably, the RGC-5 cells showed higher expression of the neuronal marker β-III tubulin and increased Thy-1-mRNA compared with the 661W cells, providing evidence of their different properties. The findings emphasize the importance of verifying the authenticity of cell lines used in ocular research and highlight the risks of contamination and altered cell properties.
摘要：
为了寻找眼部疾病的潜在途径，使用细胞系，如（视网膜神经节细胞-5）RGC-5和661W成为一个有价值的工具，包括视网膜变性和青光眼等病症.2001年，RGC-5细胞系的建立标志着青光眼研究的重大突破。随着时间的推移,然而,人们对RGC-5细胞的真实性质产生了担忧，文献中关于它们作为视网膜神经节细胞或光感受器样细胞的身份的发现相互矛盾。本研究旨在通过与661W细胞系进行比较来解决围绕RGC-5细胞系的起源和性质的争议，已知的圆锥感光模型。根据两种先前公开的再分化方案,在相同条件下使用500nM曲古抑菌素A(TSA)分化两种细胞系,并研究其形态学和神经元标记性质。结果表明，两种细胞系都是鼠类，它们表现出独特的形态学和神经元标记特性。值得注意的是,与661W细胞相比，RGC-5细胞显示神经元标志物β-III微管蛋白的更高表达和Thy-1-mRNA的增加，提供他们不同属性的证据。研究结果强调了验证眼科研究中使用的细胞系真实性的重要性，并强调了污染和细胞性质改变的风险。
