PDF(Pubmed)
Abstract：
Strain Deinococcus irradiatisoli 17bor-2 was isolated from a soil sample exposed to γ radiation at Seoul Women\'s University, Republic of Korea. The genus Deinococcus is a Gram-negative, coccus-shaped, and extremophilic bacterium, well renowned as being a radiation-resistant bacterium. Therefore, the mechanism behind the resistance to radiation and the gene responsible for the resistance could be helpful for detailed experimental studies with biotechnological applications. To study the involvement of genes in UV radiation resistance in strain 17bor-2, the genomic DNA of the strain was sequenced and constructed using the Pacific Biosciences RS II system. In addition, the complete genome sequence of strain 17bor-2 was annotated and interpreted using the Genomes-Expert Review (IMG-ER) system, along with Prodigal and JGI GenePRIMP analysis. The genome analysis of strain 17bor-2 revealed evidence of excinuclease UvrABC genes, which are key enzymes in the nucleotide excision repair (NER) mechanism, as well as genes from the recA-dependent and recQ pathways. The genome of strain Deinococcus irradiatisoli 17bor-2 was a circular chromosome comprising 3,052,043 bp with a GC content of 67.0%, including 2911 coding sequences (CDs), 49 tRNA genes, and 9 rRNA genes. In addition, their complete genome sequence annotation features provided evidence that radiation resistance genes play a central part in adaptation against extreme environmental conditions. In recent decades, excision repair genes have been indicated in considerable detail for both prokaryote and eukaryote resistance against UV-C radiation.
摘要：
从首尔女子大学暴露于γ辐射的土壤样品中分离出辐射异常球菌17bor-2菌株，大韩民国。异常球菌属是革兰氏阴性，球菌形,和极端细菌，以抗辐射细菌而闻名。因此,对辐射的抗性背后的机制和负责抗性的基因可能有助于生物技术应用的详细实验研究。为了研究基因在菌株17bor-2中的紫外线抗性中的参与，使用PacificBiosciencesRSII系统对菌株的基因组DNA进行了测序和构建。此外,使用基因组专家评论（IMG-ER）系统注释和解释菌株17bor-2的完整基因组序列，以及Produal和JGIGenePRIMP分析。菌株17bor-2的基因组分析揭示了ExcinucleaseUvrABC基因的证据，它们是核苷酸切除修复（NER）机制中的关键酶，以及recA依赖性和recQ途径的基因。辐照异常球菌17bor-2菌株的基因组为环状染色体，包含3,052,043bp，GC含量为67.0％，包括2911个编码序列（CD），49个tRNA基因，和9个rRNA基因。此外,它们完整的基因组序列注释特征提供了证据，证明抗辐射基因在适应极端环境条件中起着核心作用。近几十年来,切除修复基因对于原核生物和真核生物对UV-C辐射的抗性都有相当详细的说明。
