PDF(Pubmed)
Abstract：
Sulforaphene (SFE) is a common nutritional supplement with antibacterial, anti-cancer, and anti-inflammatory effects. However, the effects of SFE on the cariogenicity of Streptococcus mutans and dental caries have not been reported. The objectives of this study were to investigate the caries-controlling potential of SFE. The effects of SFE on S. mutans were investigated using the broth microdilution method, crystal violet staining, SEM observation, acid tolerance assays, lactic acid quantification, and polysaccharide measurements. A rat caries model was established to evaluate the caries-controlling effects and biocompatibility of SFE in vivo. SFE inhibited S. mutans growth and biofilm formation. Furthermore, SFE restrained the cariogenic properties of S. mutans, including its acid production, acid tolerance, and extracellular polysaccharide production, without affecting the bacterial viability at sub-inhibitory levels. In the rat caries model, SFE significantly arrested the onset and development of dental caries. Moreover, no visible hemolytic phenomenon or cytotoxicity was detected in the SFE groups. After four weeks of SFE treatment, all rats remained in apparent good health with no significant differences in weight gain; their hemogram and biochemical parameters were normal; no pathological changes were observed in the oral mucosa, liver, or kidneys. In conclusion, SFE was safe and inhibited the development of caries effectively.
摘要：
莱芬（SFE）是一种常见的营养补充剂，具有抗菌作用，抗癌，和抗炎作用。然而,SFE对变形链球菌和龋齿的致龋作用尚未见报道。这项研究的目的是研究SFE的龋齿控制潜力。使用肉汤微量稀释法研究了SFE对变形链球菌的影响，结晶紫染色,SEM观察,耐酸试验,乳酸定量，和多糖测量。建立大鼠龋病模型，评价SFE在体内的防龋效果和生物相容性。SFE抑制变形链球菌生长和生物膜形成。此外，SFE抑制变形链球菌的致龋特性，包括它的酸生产，耐酸性,和细胞外多糖的生产，而不影响亚抑制水平的细菌活力。在大鼠龋齿模型中，SFE显著阻止龋齿的发生和发展。此外，在SFE组中未检测到可见的溶血现象或细胞毒性。SFE治疗四周后，所有大鼠均保持明显健康，体重增加无明显差异；血象和生化指标正常；口腔粘膜无病理变化，肝脏,或肾脏。总之,SFE是安全的，有效抑制龋齿的发展。
