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Abstract：
The aim of this paper was to characterise the isotopic composition of oral squamous cell carcinoma (OSCC) specimens of different areas of the oral cavity. Secondly, we assessed whether there was a correlation between clinical stages of OSCC and isotopic abundance. The IRMS procedure was performed on 124 samples derived from 31 patients with OSCC of 15 N and 13 C to assess the isotopic composition. From each individual, four samples from the tumour, two from the margins, and two samples of healthy oral mucous membranes were derived. The two samples from the tumour and two samples from the margin were additionally subjected to histopathological assessment. Then, statistical analysis was conducted. Tumour infiltration tissues of the lower lip were characterised by higher mean δ13C values compared to samples derived from cancers of the other oral cavity regions (-23.82 ± 1.21 vs. -22.67 ± 1.35); (p = 0.04). The mean percentage of nitrogen content in tumour tissues was statistically higher in patients with the most advanced cancers (11.89 ± 0.03%) versus the group of patients with II and III stage cancers (11.12 ± 0.02%); (p = 0.04). In patients at stage IV, the mean δ13C value in the cancer samples equalled -22.69 ± 1.42 and was lower than that in patients at less severe clinical stages (p = 0.04). Lower lip cancer tissues differed in the isotopic abundance of carbon in comparison with tissues derived from the group of combined samples from other locations. Values of δ13C observed in specimens derived from lower lip cancers were similar to those observed in healthy oral mucous membranes. Cancer tissues obtained from patients in the last stage of OSCC had a different isotopic composition in comparison with those obtained from earlier stages. To confirm these observations, further research on larger groups of patients is needed.
摘要：
本文的目的是表征口腔不同区域的口腔鳞状细胞癌（OSCC）标本的同位素组成。其次,我们评估了OSCC的临床分期与同位素丰度之间是否存在相关性.对来自31名OSCC为15N和13C的患者的124个样品进行IRMS程序以评估同位素组成。从每个人身上，四个肿瘤样本，两个从边缘，并提取了两个健康口腔粘膜样本。另外对来自肿瘤的两个样品和来自边缘的两个样品进行组织病理学评估。然后，进行统计分析。与来自其他口腔区域癌症的样品相比，下唇的肿瘤浸润组织的特征是平均δ13C值较高（-23.82±1.21vs.-22.67±1.35)；(p=0.04)。与II期和III期癌症患者组（11.12±0.02％）相比，晚期癌症患者的肿瘤组织中氮含量的平均百分比在统计学上较高（11.89±0.03％）；（p=0.04）。在IV期患者中，癌症样本中的平均δ13C值等于-22.69±1.42，低于临床严重程度较低的患者（p=0.04）。与来自其他位置的组合样品组的组织相比，下唇癌组织的碳同位素丰度不同。在下唇癌标本中观察到的δ13C值与在健康口腔粘膜中观察到的值相似。与从早期阶段获得的癌组织相比，从OSCC最后阶段的患者获得的癌组织具有不同的同位素组成。为了证实这些观察，需要对更大的患者群体进行进一步的研究.
