PDF(Pubmed)
Abstract：
Bone analyses using mid-infrared spectroscopy are gaining popularity, especially with handheld spectrometers that enable on-site testing as long as the data quality meets standards. In order to diagnose Staphylococcus epidermidis in human bone grafts, this study was carried out to compare the effectiveness of the Agilent 4300 Handheld Fourier-transform infrared with the Perkin Elmer Spectrum 100 attenuated-total-reflectance infrared spectroscopy benchtop instrument. The study analyzed 40 non-infected and 10 infected human bone samples with Staphylococcus epidermidis, collecting reflectance data between 650 cm-1 and 4000 cm-1, with a spectral resolution of 2 cm-1 (Agilent 4300 Handheld) and 0.5 cm-1 (Perkin Elmer Spectrum 100). The acquired spectral information was used for spectral and unsupervised classification, such as a principal component analysis. Both methods yielded significant results when using the recommended settings and data analysis strategies, detecting a loss in bone quality due to the infection. MIR spectroscopy provides a valuable diagnostic tool when there is a tissue shortage and time is of the essence. However, it is essential to conduct further research with larger sample sizes to verify its pros and cons thoroughly.
摘要：
使用中红外光谱的骨分析越来越受欢迎，特别是手持光谱仪，只要数据质量符合标准，就可以进行现场测试。为了诊断人类骨移植物中的表皮葡萄球菌，进行这项研究是为了比较Agilent4300手持式傅立叶变换红外与PerkinElmerSpectrum100衰减全反射红外光谱台式仪器的有效性。该研究分析了40个未感染和10个感染表皮葡萄球菌的人类骨骼样本，收集650cm-1和4000cm-1之间的反射率数据，光谱分辨率为2cm-1（Agilent4300手持式）和0.5cm-1（PerkinElmerSpectrum100）。获取的光谱信息用于光谱和无监督分类，比如主成分分析。当使用推荐的设置和数据分析策略时，两种方法都产生了显著的结果，检测由于感染导致的骨质量损失。当存在组织短缺且时间至关重要时,MIR光谱学提供有价值的诊断工具。然而,必须进行更大样本量的进一步研究，以彻底验证其利弊。
