PDF(Pubmed)
Abstract：
Exploring the minimal genetic requirements for cells to maintain free living is an exciting topic in biology. Multiple approaches are employed to address the question of the minimal genome. In addition to constructing the synthetic genome in the test tube, reducing the size of the wild-type genome is a practical approach for obtaining the essential genomic sequence for living cells. The well-studied Escherichia coli has been used as a model organism for genome reduction owing to its fast growth and easy manipulation. Extensive studies have reported how to reduce the bacterial genome and the collections of genomic disturbed strains acquired, which were sufficiently reviewed previously. However, the common issue of growth decrease caused by genetic disturbance remains largely unaddressed. This mini-review discusses the considerable efforts made to improve growth fitness, which was decreased due to genome reduction. The proposal and perspective are clarified for further accumulated genetic deletion to minimise the Escherichia coli genome in terms of genome reduction, experimental evolution, medium optimization, and machine learning.
摘要：
探索细胞维持自由生活的最低遗传要求是生物学中一个令人兴奋的话题。采用多种方法来解决最小基因组的问题。除了在试管中构建合成基因组，减小野生型基因组的大小是获得活细胞必需基因组序列的实用方法。经过充分研究的大肠杆菌由于其快速生长和易于操作而被用作基因组减少的模型生物。广泛的研究已经报道了如何减少细菌基因组和基因组扰动菌株的集合，之前已经进行了充分的审查。然而,遗传干扰引起的生长减少的常见问题仍未解决。这篇小型评论讨论了为改善生长适应性所做的大量努力，由于基因组减少而减少。阐明了进一步积累的遗传缺失的建议和观点，以减少基因组减少方面的大肠杆菌基因组，实验进化,介质优化，和机器学习。
