PDF(Pubmed)
Abstract：
Feed efficiency is a major constraint in the beef industry and has a significant negative correlation with residual feed intake (RFI). RFI is widely used as a measure of feed efficiency in beef cattle and is independent of economic traits such as body weight and average daily gain. However, key traits with commonality or specificity among beef cattle breeds at the same level of RFI have not been reported. Accordingly, the present study hypothesized that signatures associated with feed efficiency would have commonality or specificity in the liver of cattle breeds at the same RFI level. By comparing and integrating liver transcriptome data, we investigated the critical signatures closely associated with RFI in beef cattle using weighted co-expression network analysis, consensus module analysis, functional enrichment analysis and protein network interaction analysis. The results showed that the consensus modules in Angus and Charolais cattle were negatively correlated, and four (turquoise, red, tan, yellow) were significantly positively correlated in Angus liver, while (turquoise, red) were significantly negatively correlated in Charolais liver. These consensus modules were found to be primarily involved in biological processes such as substance metabolism, energy metabolism and gene transcription, which may be one of the possible explanations for the difference in feed efficiency between the two beef breeds. This research also identified five key candidate genes, PLA2G12B, LCAT, MTTP, LCAT, ABCA1 and FADS1, which are closely associated with hepatic lipid metabolism. The present study has identified some modules, genes and pathways that may be the major contributors to the variation in feed efficiency among different cattle breeds, providing a new perspective on the molecular mechanisms of feed efficiency in beef cattle and a research basis for investigating molecular markers associated with feed efficiency in beef cattle.
摘要：
饲料效率是牛肉行业的主要制约因素，与剩余饲料摄入量（RFI）呈显着负相关。RFI被广泛用作肉牛饲料效率的量度，并且与体重和平均日增重等经济性状无关。然而,尚未报道在相同RFI水平的肉牛品种中具有共性或特异性的关键性状。因此，本研究假设与饲料效率相关的特征在相同RFI水平的牛品种肝脏中具有共性或特异性.通过比较和整合肝脏转录组数据，我们使用加权共表达网络分析研究了肉牛中与RFI密切相关的关键特征，共识模块分析，功能富集分析和蛋白质网络相互作用分析。结果表明，安格斯牛和夏洛莱牛的共识模块呈负相关，和四个(绿松石，红色,棕褐色,黄色)在安格斯肝脏中呈显著正相关，而(绿松石，红色)在夏洛来肝脏中呈显著负相关。发现这些共识模块主要涉及生物过程，例如物质代谢，能量代谢和基因转录，这可能是两个牛肉品种之间饲料效率差异的可能解释之一。这项研究还确定了五个关键的候选基因，PLA2G12B,LCAT,MTTP,LCAT,ABCA1和FADS1与肝脏脂质代谢密切相关。本研究已经确定了一些模块，基因和途径可能是不同牛品种之间饲料效率差异的主要贡献者，为研究肉牛饲料效率的分子机制提供了新的视角，为研究与肉牛饲料效率相关的分子标记提供了研究基础。
