PDF(Pubmed)
Abstract：
Twaite shad (Alosa fallax) includes two subspecies, the anadromous Alosa fallax nilotica, and the landlocked species Alosa fallax lacustris, representing the only Clupeidae inhabiting Lake Garda. Study of the parasitic fauna of this species in this area is still limited. For this reason, the aim of the present study was to evaluate the presence of tapeworms from A. fallax lacustris sampled in Lake Garda. Sixty-six A. fallax lacustris specimens were collected at the Milan fish market (Lombardy, Italy); specifically, an evaluation of the gastrointestinal contents was carried out for the presence of helminths. All parasites found were fixed in 70% ethanol and transferred to the laboratories of the University of Messina for morphological and molecular analysis. Parts of the parasites were stained with Semichon\'s carmine red technique. Molecular analysis was carried out using LSU rRNA and ITS2 region genes. Eight specimens out of sixty-six (12.1%) were positive for adult cestodes within the pyloric caeca. Morphological and molecular analysis could to identify the parasites found to be Proteocephalus longicollis. This parasite species is not considered a zoonotic agent, representing a low risk of parasitic fish-borne zoonosis for consumers of this appreciated fish from Lake Garda.
摘要：
Twaiteshad（Alosafallax）包括两个亚种，无性系Alosaflaxnilotica,和内陆物种Alosafallaxlacustris，代表唯一居住在加尔达湖的Clupeidae。对该地区该物种寄生动物的研究仍然有限。出于这个原因,本研究的目的是评估加尔达湖采样的A.fallaxlacustris虫的存在。在米兰鱼市场（伦巴第，意大利)；具体来说，对胃肠道内容物是否存在蠕虫进行评估。所有发现的寄生虫都固定在70％的乙醇中，并转移到墨西哥大学的实验室进行形态和分子分析。部分寄生虫用Semichon的胭脂红技术染色。使用LSUrRNA和ITS2区基因进行分子分析。在幽门盲肠内的66个标本中，有8个（12.1％）对成虫呈阳性。形态和分子分析可以确定发现的寄生虫是长长颈变形虫。这种寄生虫物种不被认为是人畜共患病原体，对于来自加尔达湖的这种赞赏鱼的消费者来说，寄生虫传播的人畜共患病的风险较低。
