PDF(Pubmed)
Abstract：
There is growing interest in the application of fecal microbiota transplants (FMTs) in small animal medicine, but there are few published studies that have tested their effects in the domestic cat (Felis catus). Here we use 16S rRNA gene sequencing to examine fecal microbiome changes in 46 domestic cats with chronic digestive issues that received FMTs using lyophilized stool that was delivered in oral capsules. Fecal samples were collected from FMT recipients before and two weeks after the end of the full course of 50 capsules, as well as from their stool donors (N = 10), and other healthy cats (N = 113). The fecal microbiomes of FMT recipients varied with host clinical signs and dry kibble consumption, and shifts in the relative abundances of Clostridium, Collinsella, Megamonas, Desulfovibrio and Escherichia were observed after FMT. Overall, donors shared 13% of their bacterial amplicon sequence variants (ASVs) with FMT recipients and the most commonly shared ASVs were classified as Prevotella 9, Peptoclostridium, Bacteroides, and Collinsella. Lastly, the fecal microbiomes of cats with diarrhea became more similar to the microbiomes of age-matched and diet-matched healthy cats compared to cats with constipation. Overall, our results suggest that microbiome responses to FMT may be modulated by the FMT recipient\'s initial presenting clinical signs, diet, and their donor\'s microbiome.
摘要：
人们对粪便微生物移植（FMT）在小动物医学中的应用越来越感兴趣，但是很少有发表的研究测试了它们在家猫（Feliscatus）中的作用。在这里，我们使用16SrRNA基因测序来检查46只患有慢性消化问题的家猫的粪便微生物组变化，这些猫使用口服胶囊递送的冻干粪便接受FMT。在50粒胶囊的整个疗程结束前和两周后，从FMT接受者那里收集粪便样本，以及他们的粪便捐赠者（N=10），和其他健康的猫（N=113）。FMT接受者的粪便微生物组随宿主临床症状和干粗粒消耗而变化，以及梭菌相对丰度的变化，Collinsella,Megamonas,FMT后观察到脱硫弧菌和埃希氏菌。总的来说,供体与FMT受体共享13％的细菌扩增子序列变体（ASV），最常见的ASV被分类为Prevotella9，拟杆菌,还有Collinsella.最后,与便秘猫相比，腹泻猫的粪便微生物组变得与年龄匹配和饮食匹配的健康猫的微生物组更相似。总的来说,我们的结果表明，对FMT的微生物组反应可能受FMT接受者最初出现的临床体征的调节，饮食，和他们的捐赠者的微生物组。
