PDF(Pubmed)
Abstract：
Migraine is a common primary headache disorder with both environmental and genetic inputs. Cumulative evidence indicates an association between vitamin D and headache. Unravelling the precise role of vitamin D and its receptor in the pathophysiology of migraine can eventually contribute to more efficient prevention and management of this headache disorder. The aim of the study was to investigate the relation of the three most studied VDR variants, i.e., FokI (rs2228570), TaqI (rs731236) and BsmI (rs1544410), with migraine susceptibility and distinct clinical phenotypes in a Southeastern European case-control population residing in Greece. DNA was extracted from 191 unrelated patients diagnosed with migraine and 265 headache-free controls and genotyped using real-time PCR (LightSNiP assays) followed by melting curve analysis. Genotype frequency distribution analysis of the TaqI and BsmI variants showed a statistically significant difference between migraine cases and controls. In addition, subgroup analyses revealed a significant association between all three studied VDR variants, particularly with a migraine without aura subtype. Therefore, the current study provides supporting evidence for a possible association of VDR variants with migraines, particularly migraine without aura susceptibility in Southeastern Europeans residing in Greece, further reinforcing the emerging role of vitamin D and its receptor in migraines.
摘要：
偏头痛是一种常见的原发性头痛疾病，具有环境和遗传输入。累积证据表明维生素D与头痛之间存在关联。揭示维生素D及其受体在偏头痛病理生理学中的确切作用最终可以有助于更有效地预防和管理这种头痛疾病。该研究的目的是调查三个研究最多的VDR变体之间的关系，即，FokI(rs2228570),TaqI(rs731236)和BsmI(rs1544410)，在居住在希腊的东南欧病例对照人群中，偏头痛易感性和不同的临床表型。从191名诊断为偏头痛的无关患者和265名无头痛对照中提取DNA，并使用实时PCR（LightSNiP测定）进行基因分型，然后进行熔解曲线分析。TaqI和BsmI变体的基因型频率分布分析显示，偏头痛病例和对照组之间存在统计学上的显着差异。此外,亚组分析显示，所有三个研究的VDR变异体之间存在显著关联，特别是没有先兆亚型的偏头痛。因此,本研究为VDR变异与偏头痛的可能关联提供了支持证据,特别是在居住在希腊的东南欧人没有先兆的偏头痛，进一步加强维生素D及其受体在偏头痛中的新兴作用。
