PDF(Pubmed)
Abstract：
Sargassum horneri (SH) and Ulva australis (UA) are marine waste resources that cause environmental and economic problems when entering or multiplying the coastal waters of Jeju Island. We analyzed their anti-diabetic efficacy to assess their reusability as functional additives. The alpha-glucosidase inhibitory activity of SH and UA extracts was confirmed, and the effect of UA extract was higher than that of SH. After the induction of insulin-resistant HepG2 cells, the effects of the two marine extracts on oxidative stress, intracellular glucose uptake, and glycogen content were compared to the positive control, metformin. Treatment of insulin-resistant HepG2 cells with SH and UA resulted in a concentration-dependent decrease in oxidative stress and increased intracellular glucose uptake and glycogen content. Moreover, SH and UA treatment upregulated the expression of IRS-1, AKT, and GLUT4, which are suppressed in insulin resistance, to a similar degree to metformin, and suppressed the expression of FoxO1, PEPCK involved in gluconeogenesis, and GSK-3β involved in glycogen metabolism. The oral administration of these extracts to rats with streptozotocin-induced diabetes led to a higher weight gain than that in the diabetic group. Insulin resistance and oral glucose tolerance are alleviated by the regulation of blood glucose. Thus, the SH and UA extracts may be used in the development of therapeutic agents or supplements to improve insulin resistance.
摘要：
Sargassumhorneri（SH）和Ulvaaustralis（UA）是海洋废物资源，在进入或繁殖济州岛沿海水域时会引起环境和经济问题。我们分析了它们的抗糖尿病功效，以评估其作为功能性添加剂的可重用性。证实了SH和UA提取物的α-葡萄糖苷酶抑制活性，且UA提取物的药效高于SH。胰岛素抵抗HepG2细胞诱导后,两种海洋提取物对氧化应激的影响，细胞内葡萄糖摄取，和糖原含量与阳性对照进行比较，二甲双胍。用SH和UA处理胰岛素抵抗的HepG2细胞导致氧化应激的浓度依赖性降低，细胞内葡萄糖摄取和糖原含量增加。此外，SH和UA处理上调IRS-1、AKT、和GLUT4，在胰岛素抵抗中被抑制，与二甲双胍的程度相似，并抑制参与糖异生的FoxO1，PEPCK的表达，和GSK-3β参与糖原代谢。将这些提取物口服给链脲佐菌素诱导的糖尿病大鼠导致比糖尿病组更高的体重增加。胰岛素抵抗和口服葡萄糖耐量可通过调节血糖得到缓解。因此，SH和UA提取物可用于开发改善胰岛素抵抗的治疗剂或补充剂。
