Abstract：
Investigation of aminoglycoside acetyltransferases in actinobacteria of the genus Streptomyces is an integral part of the study of soil bacteria as the main reservoir and possible source of drug resistance genes. Previously, we have identified and biochemically characterized three aminoglycoside phosphotransferases, which cause resistance to kanamycin, neomycin, paromomycin, streptomycin, and hygromycin B in the strain Streptomyces rimosus ATCC 10970 (producing oxytetracycline), which is resistant to most natural aminoglycoside antibiotics. In the presented work, it was shown that the resistance of this strain to other AGs is associated with the presence of the enzyme aminoglycoside acetyltransferase, belonging to the AAC(2\') subfamily. Induction of the expression of the gene, designated by us as aac(2\')-If, in Escherichia coli cells determines resistance to a wide range of natural aminoglycoside antibiotics (neomycin, gentamicin, tobramycin, sisomycin, and paromomycin) and increases minimum inhibitory concentrations of these antibiotics.
摘要：
研究链霉菌属放线菌中的氨基糖苷乙酰转移酶是研究土壤细菌作为主要储库和可能的耐药基因来源的重要组成部分。以前,我们已经鉴定并生化表征了三种氨基糖苷磷酸转移酶，导致对卡那霉素的抗药性，新霉素,巴龙霉素,链霉素,和潮霉素B在菌株链霉菌ATCC10970（产生土霉素）中，对大多数天然氨基糖苷类抗生素具有抗性。在提交的作品中，结果表明，该菌株对其他AG的抗性与氨基糖苷乙酰转移酶的存在有关，属于AAC（2\'）亚科。基因表达的诱导，由我们指定为aac(2\')-如果，在大肠杆菌细胞中确定对多种天然氨基糖苷类抗生素（新霉素，庆大霉素,妥布霉素,西索霉素,和巴龙霉素），并增加这些抗生素的最低抑制浓度。
