Abstract：
This study aimed to evaluate the levels of tumour necrosis factor-alpha (TNF-α), interleukin-1beta (IL-1β) and interferon-gamma (IFN-γ) with asymptomatic apical periodontitis (AP). A total of 60 participants were randomly divided into two groups: the conventional irrigation (control) and the Nd: YAG laser irradiation. The interstitial fluids were obtained after root canal cleaning (day 0) and 1 week later (day 7). The TNF-α, IL-1β and IFN-γ levels were assayed using the enzyme-linked immunosorbent assay. The Mann-Whitney U, continuity correction chi-square, Pearson chi-square and Fisher exact tests were used. An increased level of cytokines on day 7 in the control group was observed, without statistically significant differences (p > 0.05). All cytokine levels decreased over time in the laser group. Only the IL-1β level showed a significant difference (p < 0.05). Nd: YAG irradiation has a positive effect on decreasing the proinflammatory cytokine level and may help to control infection in teeth with AP.
摘要：
本研究旨在评估肿瘤坏死因子-α（TNF-α）的水平，白细胞介素-1β（IL-1β）和干扰素-γ（IFN-γ）与无症状根尖周炎（AP）。将60名参与者随机分为两组：常规灌溉（对照）和Nd：YAG激光照射。在根管清洁后(第0天)和1周后(第7天)获得间质液。TNF-α,使用酶联免疫吸附测定法测定IL-1β和IFN-γ水平。Mann-WhitneyU,连续性校正卡方，使用Pearson卡方检验和Fisher精确检验。观察到对照组第7天的细胞因子水平升高，差异无统计学意义(p>0.05)。在激光组中,所有细胞因子水平随时间降低。仅IL-1β水平显示显著差异(p<0.05)。Nd：YAG照射对降低促炎细胞因子水平具有积极作用，并可能有助于控制AP牙齿的感染。
