关键词: Bacteriophage Genomic characterization and Phylogenetic analysis Host range Jumbo phage Phage characterization Phage isolation Phage stability

Mesh : Humans Anti-Bacterial Agents Bacteriophages Genomics Public Health

来  源:   DOI:10.1016/bs.pmbts.2023.03.008

Abstract:
Bacterial resistance threatens public health due to a lack of novel antibacterial classes since the 21st century. Bacteriophages, the most ubiquitous microorganism on Earth and natural predators of bacteria, have the potential to save the world from the post-antibiotic era. Therefore, phage isolation and characterization are in high demand to find suitable phages for therapeutic and bacterial control applications. The chapter presents brief guidance supported by recommendations on the isolation of phages, and initial screening of phage antimicrobial efficacy, in addition to, conducting comprehensive characterization addressing morphological, biological, genomic, and taxonomic features.
摘要:
自21世纪以来,由于缺乏新型抗菌类别,细菌耐药性威胁着公众健康。噬菌体,地球上最普遍的微生物和细菌的天敌,有可能从后抗生素时代拯救世界。因此,对噬菌体分离和表征的要求很高,以找到用于治疗和细菌控制应用的合适噬菌体。本章提供了简短的指导,并提出了有关噬菌体隔离的建议,和噬菌体抗菌功效的初步筛选,此外,进行全面的表征,寻址形态学,生物,基因组,和分类学特征。
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