PDF(Pubmed)
Abstract：
Cockroach microbiome studies generally focus on pest cockroach species belonging to the Blattidae and Ectobiidae families. There are no reports characterizing the gut microbiome of non-pest cockroach species Blaberus discoidalis (family Blaberidae), which is commonly used as a food source for insectivorous animals. We discovered the parasitic nematode Leidynema appendiculata in the B. discoidalis hindgut during initial work characterizing the gut microbiome of this organism. To determine the proportion of the B. discoidalis colony that was colonized by L. appendiculata, 28 S rDNA was amplified using two Methods: endpoint polymerase chain reaction (PCR) and loop-mediated isothermal amplification (LAMP). B. discoidalis colonies were raised on three diet types (control, high fibre, and high fat and salt) for 21 days before dissection. Each individual was sexed during dissection to identify potential sex-based effects of colonization. Data collected were analysed to determine if diet and sex impacted parasite colonization patterns. LAMP detected a higher proportion of parasite positive samples when compared to endpoint PCR. No sex- or diet-based differences in L. appendiculata colonization were found. This study adds to the limited existing knowledge of the B. discoidalis gut microbiome.
摘要：
蟑螂微生物组研究通常集中在属于Blattidae和Ectobiidae家族的害虫蟑螂物种上。没有报道描述非害虫蟑螂物种盘状夜蛾（Blaberidae家族）的肠道微生物组，通常用作食虫动物的食物来源。在表征该生物的肠道微生物组的初始工作中，我们在盘状芽孢杆菌后肠中发现了寄生线虫。为了确定阑尾乳杆菌定植的盘状芽孢杆菌菌落的比例，使用两种方法扩增28SrDNA:终点聚合酶链反应(PCR)和环介导等温扩增(LAMP)。盘状芽孢杆菌菌落在三种饮食类型(对照，高纤维，和高脂肪和盐）在解剖前21天。在解剖过程中对每个人进行性别鉴定，以确定潜在的基于性别的定殖效应。分析收集的数据以确定饮食和性别是否影响寄生虫定植模式。当与终点PCR相比时,LAMP检测到更高比例的寄生虫阳性样品。没有发现阑尾乳杆菌定植的基于性别或饮食的差异。这项研究增加了对盘状芽孢杆菌肠道微生物组的有限现有知识。
