PDF(Pubmed)
Abstract：
BACKGROUND: Multinucleated giant cells (MGCs) in bladder carcinomas are poorly studied.
OBJECTIVE: To describe the function, morphogenesis, and origin of mononuclear and MGCs in urothelial carcinoma (UC) of the bladder in Bulgarian and French patients.
METHODS: Urothelial bladder carcinomas (n = 104) from 2016-2020 were analyzed retrospectively using immunohistochemical (IHC) and histochemical stain examination. Giant cells in the bladder stroma were found in 35.6% of cases, more often in high-grades.
RESULTS: We confirm that MGCs in the mucosa in UC of the bladder were positive for both mesenchymal and myofibroblast markers (vimentin, smooth muscle actin, Desmin, and CD34) and the macrophage marker CD68. Furthermore, IHC studies revealed the following profile of these cells: Positive for p16; negative for epithelial (CK AE1/AE3 and GATA-3), vascular (CD31), neural (PS100 and C-KIT), cambial, blastic (CD34-blasts and C-KIT), and immune markers (IG G, immunoglobulin G4, and PD-L1); no proliferative activity, possess no specific immune function, and cannot be used to calculate the Combined Positive Score scale.
CONCLUSIONS: In conclusion, the giant stromal cells in non-tumor and tumor bladder can be used as a characteristic and relatively constant, although nonspecific, histological marker for chronic bladder damage, reflecting the chronic irritation or inflammation. Likewise, according to the morphological and IHC of the mono- and multinucleated giant cells in the bladder, they are most likely represent telocytes capable of adapting their morphology to the pathology of the organ.
OBJECTIVE: To describe the function, morphogenesis, and origin of mononuclear and MGCs in urothelial carcinoma (UC) of the bladder in Bulgarian and French patients.
METHODS: Urothelial bladder carcinomas (n = 104) from 2016-2020 were analyzed retrospectively using immunohistochemical (IHC) and histochemical stain examination. Giant cells in the bladder stroma were found in 35.6% of cases, more often in high-grades.
RESULTS: We confirm that MGCs in the mucosa in UC of the bladder were positive for both mesenchymal and myofibroblast markers (vimentin, smooth muscle actin, Desmin, and CD34) and the macrophage marker CD68. Furthermore, IHC studies revealed the following profile of these cells: Positive for p16; negative for epithelial (CK AE1/AE3 and GATA-3), vascular (CD31), neural (PS100 and C-KIT), cambial, blastic (CD34-blasts and C-KIT), and immune markers (IG G, immunoglobulin G4, and PD-L1); no proliferative activity, possess no specific immune function, and cannot be used to calculate the Combined Positive Score scale.
CONCLUSIONS: In conclusion, the giant stromal cells in non-tumor and tumor bladder can be used as a characteristic and relatively constant, although nonspecific, histological marker for chronic bladder damage, reflecting the chronic irritation or inflammation. Likewise, according to the morphological and IHC of the mono- and multinucleated giant cells in the bladder, they are most likely represent telocytes capable of adapting their morphology to the pathology of the organ.
摘要：
背景：对膀胱癌中的多核巨细胞（MGCs）研究甚少。
目的：为了描述功能，形态发生,保加利亚和法国患者膀胱尿路上皮癌（UC）中单核和MGCs的起源。
方法：对2016-2020年膀胱尿路上皮癌（n=104）进行回顾性分析，采用免疫组织化学（IHC）和组织化学染色检查。35.6%的病例发现膀胱间质巨细胞,更常见的是高年级。
结果：我们证实，膀胱UC粘膜中的MGCs对间充质和肌成纤维细胞标志物均呈阳性（波形蛋白，平滑肌肌动蛋白，Desmin,和CD34）和巨噬细胞标记CD68。此外，IHC研究揭示了这些细胞的以下特征：p16阳性；上皮阴性（CKAE1/AE3和GATA-3），血管（CD31），神经（PS100和C-KIT），形成层,blastic（CD34-blast和C-KIT），和免疫标记(IGG，免疫球蛋白G4和PD-L1）；无增殖活性，没有特定的免疫功能，并且不能用于计算组合阳性评分量表。
结论：结论：非肿瘤和肿瘤膀胱中的巨大间质细胞可以作为特征性和相对恒定的，虽然没有特异性，慢性膀胱损伤的组织学标志物，反映慢性刺激或炎症。同样，根据膀胱中单核和多核巨细胞的形态学和IHC，它们很可能代表能够使其形态适应器官病理的末端细胞。
目的：为了描述功能，形态发生,保加利亚和法国患者膀胱尿路上皮癌（UC）中单核和MGCs的起源。
方法：对2016-2020年膀胱尿路上皮癌（n=104）进行回顾性分析，采用免疫组织化学（IHC）和组织化学染色检查。35.6%的病例发现膀胱间质巨细胞,更常见的是高年级。
结果：我们证实，膀胱UC粘膜中的MGCs对间充质和肌成纤维细胞标志物均呈阳性（波形蛋白，平滑肌肌动蛋白，Desmin,和CD34）和巨噬细胞标记CD68。此外，IHC研究揭示了这些细胞的以下特征：p16阳性；上皮阴性（CKAE1/AE3和GATA-3），血管（CD31），神经（PS100和C-KIT），形成层,blastic（CD34-blast和C-KIT），和免疫标记(IGG，免疫球蛋白G4和PD-L1）；无增殖活性，没有特定的免疫功能，并且不能用于计算组合阳性评分量表。
结论：结论：非肿瘤和肿瘤膀胱中的巨大间质细胞可以作为特征性和相对恒定的，虽然没有特异性，慢性膀胱损伤的组织学标志物，反映慢性刺激或炎症。同样，根据膀胱中单核和多核巨细胞的形态学和IHC，它们很可能代表能够使其形态适应器官病理的末端细胞。
