Abstract：
Long non-coding RNAs (lncRNAs) act as competing endogenous RNAs (ceRNAs) that play a significant role in bovine embryo development; but the influence of sperm-borne lncRNA on the preimplantation development of bovine embryos has not been reported in detail. In this study, we aimed to clarify how sperm-borne lncRNAs can act to regulate early development of bovine embryos. Utilizing high-throughput sequencing technology and quantitative real-time PCR (qPCR), we found that the lncRNA, loc100847420, was highly enriched in bovine sperm and was carried into the oocyte during fertilization. Introduction of wild-type loc100847420 had no effect on cleavage rate of parthenogenetic embryos compared with injection of mutant loc100847420 (70.58 ± 2.85% vs 70.46 ± 1.98%, p > 0.05), but significantly improved the blastocyst rate (33.67 ± 2.40% vs 28.35 ± 3.06%, p < 0.05), total numbers of cells (p < 0.05), numbers of inner cell mass (ICM) cells (p < 0.05) and numbers of trophoblast (TE) cells (p < 0.05). In summary, the sperm-borne lncRNA, loc100847420, can improve the developmental potential of early bovine embryos.
摘要：
长链非编码RNA（lncRNAs）作为竞争性内源性RNA（ceRNAs）在牛胚胎发育中起重要作用；但是精子携带的lncRNA对牛胚胎植入前发育的影响尚未详细报道。在这项研究中,我们旨在阐明精子携带的lncRNAs如何调节牛胚胎的早期发育。利用高通量测序技术和实时定量PCR(qPCR),我们发现lncRNA，loc100847420在牛精子中高度富集，并在受精过程中被带入卵母细胞。与注射突变体loc100847420相比，野生型loc100847420的引入对孤雌生殖胚胎的卵裂率没有影响（70.58±2.85％vs70.46±1.98％，P>0.05)，但显著提高了囊胚率(33.67±2.40%vs28.35±3.06%,p<0.05)，细胞总数（p<0.05），内细胞团（ICM）细胞数（p<0.05）和滋养层（TE）细胞数（p<0.05）。总之,精子携带的lncRNA，loc100847420,可以提高早期牛胚胎的发育潜能。
