UNASSIGNED: This study was carried out on 40 cases with BD and 40 healthy control subjects. The collection of serum samples was done before performing a real-time PCR to estimate the relative gene expression of ATG1, AKT, miRNA-155, mTOR, TAB2, and circRNA-0067835. Additionally, IL-1β, IL-17, and TNF-α serum levels were determined by ELISA.
UNASSIGNED: Behcet Disease (BD) patients had significantly upregulated circRNA-0067835, with subsequent downregulation of its target gene, miRNA-155 than controls (P<0.05). In addition, decreased miRNA-155 gene expression was correlated with significantly increased TAB2 gene expression levels in BD patients compared to the controls (P<0.05). Furthermore, enhanced production of pro-inflammatory cytokines was detected in cases with BD than in controls.
UNASSIGNED: The correlation between circRNA-0067835 and miRNA-155 fairly contributes to the regulation of cytokine production in BD via the modulation of autophagy. The investigation of the circRNA-0067835 and the microRNA-155 and their downstream adaptor molecules could be a potential therapeutic agent for BD.
■这项研究是对40例BD患者和40例健康对照受试者进行的。在进行实时PCR以估计ATG1、AKT、miRNA-155,mTOR,TAB2和circRNA-0067835。此外,IL-1β,ELISA法测定血清IL-17和TNF-α水平。
■Behcet病(BD)患者的circRNA-0067835显著上调,随后其靶基因下调,miRNA-155高于对照(P<0.05)。此外,与对照组相比,BD患者miRNA-155基因表达水平降低与TAB2基因表达水平显著升高相关(P<0.05)。此外,与对照组相比,在BD患者中检测到促炎细胞因子的产生增加.
■circRNA-0067835和miRNA-155之间的相关性相当有助于通过调节自噬来调节BD中细胞因子的产生。对circRNA-0067835和microRNA-155及其下游接头分子的研究可能是BD的潜在治疗剂。