PDF(Pubmed)
Abstract：
UNASSIGNED: Autophagy has been proven to contribute to maintaining eukaryotic cells\' normal intracellular homeostasis, whereas autophagy malfunction may predispose to Behcet Disease (BD). The accumulation of the products of autophagic degradation as well as impairment in autophagic flux in cases with BD, may be attributed to dysregulated miRNA-155 expression. This study attempts to determine the contribution of circRNA-0067835 in miRNA-155-mediated modulation of the autophagy axis as well as to investigate its impact on the production of pro-inflammatory cytokines in BD.
UNASSIGNED: This study was carried out on 40 cases with BD and 40 healthy control subjects. The collection of serum samples was done before performing a real-time PCR to estimate the relative gene expression of ATG1, AKT, miRNA-155, mTOR, TAB2, and circRNA-0067835. Additionally, IL-1β, IL-17, and TNF-α serum levels were determined by ELISA.
UNASSIGNED: Behcet Disease (BD) patients had significantly upregulated circRNA-0067835, with subsequent downregulation of its target gene, miRNA-155 than controls (P<0.05). In addition, decreased miRNA-155 gene expression was correlated with significantly increased TAB2 gene expression levels in BD patients compared to the controls (P<0.05). Furthermore, enhanced production of pro-inflammatory cytokines was detected in cases with BD than in controls.
UNASSIGNED: The correlation between circRNA-0067835 and miRNA-155 fairly contributes to the regulation of cytokine production in BD via the modulation of autophagy. The investigation of the circRNA-0067835 and the microRNA-155 and their downstream adaptor molecules could be a potential therapeutic agent for BD.
UNASSIGNED: This study was carried out on 40 cases with BD and 40 healthy control subjects. The collection of serum samples was done before performing a real-time PCR to estimate the relative gene expression of ATG1, AKT, miRNA-155, mTOR, TAB2, and circRNA-0067835. Additionally, IL-1β, IL-17, and TNF-α serum levels were determined by ELISA.
UNASSIGNED: Behcet Disease (BD) patients had significantly upregulated circRNA-0067835, with subsequent downregulation of its target gene, miRNA-155 than controls (P<0.05). In addition, decreased miRNA-155 gene expression was correlated with significantly increased TAB2 gene expression levels in BD patients compared to the controls (P<0.05). Furthermore, enhanced production of pro-inflammatory cytokines was detected in cases with BD than in controls.
UNASSIGNED: The correlation between circRNA-0067835 and miRNA-155 fairly contributes to the regulation of cytokine production in BD via the modulation of autophagy. The investigation of the circRNA-0067835 and the microRNA-155 and their downstream adaptor molecules could be a potential therapeutic agent for BD.
摘要：
■自噬已被证明有助于维持真核细胞正常的细胞内稳态，而自噬功能异常可能易患白塞病（BD）。在BD的情况下，自噬降解产物的积累以及自噬通量的损害，可能归因于miRNA-155表达失调。这项研究试图确定circRNA-0067835在miRNA-155介导的自噬轴调节中的作用，并研究其对BD中促炎细胞因子产生的影响。
■这项研究是对40例BD患者和40例健康对照受试者进行的。在进行实时PCR以估计ATG1、AKT、miRNA-155,mTOR,TAB2和circRNA-0067835。此外,IL-1β,ELISA法测定血清IL-17和TNF-α水平。
■Behcet病（BD）患者的circRNA-0067835显著上调，随后其靶基因下调，miRNA-155高于对照(P<0.05)。此外,与对照组相比,BD患者miRNA-155基因表达水平降低与TAB2基因表达水平显著升高相关(P<0.05)。此外，与对照组相比,在BD患者中检测到促炎细胞因子的产生增加.
■circRNA-0067835和miRNA-155之间的相关性相当有助于通过调节自噬来调节BD中细胞因子的产生。对circRNA-0067835和microRNA-155及其下游接头分子的研究可能是BD的潜在治疗剂。
■这项研究是对40例BD患者和40例健康对照受试者进行的。在进行实时PCR以估计ATG1、AKT、miRNA-155,mTOR,TAB2和circRNA-0067835。此外,IL-1β,ELISA法测定血清IL-17和TNF-α水平。
■Behcet病（BD）患者的circRNA-0067835显著上调，随后其靶基因下调，miRNA-155高于对照(P<0.05)。此外,与对照组相比,BD患者miRNA-155基因表达水平降低与TAB2基因表达水平显著升高相关(P<0.05)。此外，与对照组相比,在BD患者中检测到促炎细胞因子的产生增加.
■circRNA-0067835和miRNA-155之间的相关性相当有助于通过调节自噬来调节BD中细胞因子的产生。对circRNA-0067835和microRNA-155及其下游接头分子的研究可能是BD的潜在治疗剂。
