PDF(Pubmed)
Abstract：
Inflammasomes are a group of molecules that are strongly involved in causing inflammation. This study aimed to evaluate the expression of NLR family pyrin domain containing 1 (NLRP1), NLRP3, and Apoptosis-associated speck-like protein containing a CARD (ASC) as well as their association with serum level of interleukin (IL)-1β in patients with coronavirus disease 2019 (COVID-19).
Thirty COVID-19 patients and 30 healthy subjects (HS) were recruited. Peripheral blood specimens were collected from subjects to assess NLRP1, NLRP3, and ASC gene expression by Real time-PCR technique. Serum levels of IL-1β were also measured via the enzyme-linked immunosorbent assay (ELISA).
The findings showed no significant differences in serum IL-1β level between COVID-19 patients and the HS group. mRNA expression of ASC (P = 0.008) and NLRP1 (P = 0.03) gene had a significant increase in COVID-19 patients compared to HS, while there was no significant increase in the expression of NLRP3 between the studied group. There were significant correlations between patient\'s data and expression levels of NLRP1, NLRP3, IL-1β, and ACS.
NLRP1 and ASC may have a more critical role in the generation of the active form of IL-1β in COVID-19 patients compared to NLRP3. However, serum levels of IL-1β in patients did not show a significant increase, which may be due to the patient\'s condition and the application of virus escape mechanisms through impaired NLRP3 expression and its malfunction.
Thirty COVID-19 patients and 30 healthy subjects (HS) were recruited. Peripheral blood specimens were collected from subjects to assess NLRP1, NLRP3, and ASC gene expression by Real time-PCR technique. Serum levels of IL-1β were also measured via the enzyme-linked immunosorbent assay (ELISA).
The findings showed no significant differences in serum IL-1β level between COVID-19 patients and the HS group. mRNA expression of ASC (P = 0.008) and NLRP1 (P = 0.03) gene had a significant increase in COVID-19 patients compared to HS, while there was no significant increase in the expression of NLRP3 between the studied group. There were significant correlations between patient\'s data and expression levels of NLRP1, NLRP3, IL-1β, and ACS.
NLRP1 and ASC may have a more critical role in the generation of the active form of IL-1β in COVID-19 patients compared to NLRP3. However, serum levels of IL-1β in patients did not show a significant increase, which may be due to the patient\'s condition and the application of virus escape mechanisms through impaired NLRP3 expression and its malfunction.
摘要：
背景：炎性体是一组强烈参与引起炎症的分子。本研究旨在评估NLR家族pyrin结构域1（NLRP1）的表达，NLRP3和含有CARD（ASC）的凋亡相关斑点样蛋白，以及它们与2019年冠状病毒病患者血清白介素（IL）-1β水平的关系（COVID-19）。
方法：招募30例COVID-19患者和30例健康受试者（HS）。收集受试者的外周血标本，通过实时PCR技术评估NLRP1，NLRP3和ASC基因的表达。还通过酶联免疫吸附测定（ELISA）测量了IL-1β的血清水平。
结果：结果显示COVID-19患者与HS组血清IL-1β水平无明显差异。与HS相比，COVID-19患者ASC（P=0.008）和NLRP1（P=0.03）基因的mRNA表达明显增加，而NLRP3的表达在研究组之间没有显着增加。患者数据与NLRP1、NLRP3、IL-1β、ACS。
结论：与NLRP3相比，NLRP1和ASC在COVID-19患者IL-1β活性形式的产生中可能具有更关键的作用。然而,患者的血清IL-1β水平没有显着升高，这可能是由于患者的病情和通过受损的NLRP3表达及其功能障碍的病毒逃逸机制的应用。
方法：招募30例COVID-19患者和30例健康受试者（HS）。收集受试者的外周血标本，通过实时PCR技术评估NLRP1，NLRP3和ASC基因的表达。还通过酶联免疫吸附测定（ELISA）测量了IL-1β的血清水平。
结果：结果显示COVID-19患者与HS组血清IL-1β水平无明显差异。与HS相比，COVID-19患者ASC（P=0.008）和NLRP1（P=0.03）基因的mRNA表达明显增加，而NLRP3的表达在研究组之间没有显着增加。患者数据与NLRP1、NLRP3、IL-1β、ACS。
结论：与NLRP3相比，NLRP1和ASC在COVID-19患者IL-1β活性形式的产生中可能具有更关键的作用。然而,患者的血清IL-1β水平没有显着升高，这可能是由于患者的病情和通过受损的NLRP3表达及其功能障碍的病毒逃逸机制的应用。
