Abstract：
OBJECTIVE: The purpose of this case series was to describe the effect of autologous PRF membrane for corneal reconstruction surgery in dogs. PRF membranes made from two healthy dogs unrelated to the current case series were used for PRF histologic analyses.
METHODS: Seven dogs with complicated corneal ulcerations.
METHODS: A complete ophthalmic examination, hematology, and fibrinogen analysis were performed pre-surgery. A PRF clot was made from autologous blood in a serum tube after centrifugation in a horizontal Bio-PRF® Centrifuge at 700 × g for 8 min. The PRF clot was processed in a PRF-Box® into a PRF membrane. The PRF membrane was sutured to the corneal ulcer bed. Each dog had a follow-up at days 5-7, 12-14, and 30-40 post-surgery. A final long-term follow-up was performed as well.
RESULTS: A positive outcome with healing and a \"good\" quality PRF membrane was seen in six out of seven dogs. One dog had a fibrinogen level below normal range and the PRF membrane was of \"poor\" quality. This dog developed a descemetocele 13 days post-surgery and needed rescue surgery. Mean healing time for all dogs was 9 ± 5.5 days. Minimal scarring, corneal pigmentation, and vascularization were observed at the final long-term follow-up 288 ± 44 days post-surgery.
CONCLUSIONS: PRF membrane was successful as graft material for corneal ulceration reconstruction surgery. Low fibrinogen appeared to have negative effect on the quality of the PRF membrane, showing the importance for the surgeon to evaluate the quality of the PRF membrane prior to surgery.
METHODS: Seven dogs with complicated corneal ulcerations.
METHODS: A complete ophthalmic examination, hematology, and fibrinogen analysis were performed pre-surgery. A PRF clot was made from autologous blood in a serum tube after centrifugation in a horizontal Bio-PRF® Centrifuge at 700 × g for 8 min. The PRF clot was processed in a PRF-Box® into a PRF membrane. The PRF membrane was sutured to the corneal ulcer bed. Each dog had a follow-up at days 5-7, 12-14, and 30-40 post-surgery. A final long-term follow-up was performed as well.
RESULTS: A positive outcome with healing and a \"good\" quality PRF membrane was seen in six out of seven dogs. One dog had a fibrinogen level below normal range and the PRF membrane was of \"poor\" quality. This dog developed a descemetocele 13 days post-surgery and needed rescue surgery. Mean healing time for all dogs was 9 ± 5.5 days. Minimal scarring, corneal pigmentation, and vascularization were observed at the final long-term follow-up 288 ± 44 days post-surgery.
CONCLUSIONS: PRF membrane was successful as graft material for corneal ulceration reconstruction surgery. Low fibrinogen appeared to have negative effect on the quality of the PRF membrane, showing the importance for the surgeon to evaluate the quality of the PRF membrane prior to surgery.
摘要：
目的：本病例系列的目的是描述自体PRF膜在犬角膜重建手术中的作用。由与当前病例系列无关的两只健康狗制成的PRF膜用于PRF组织学分析。
方法：7只患有复杂角膜溃疡的狗。
方法：完整的眼科检查，血液学,术前进行纤维蛋白原分析。在水平Bio-PRF®离心机中以700xg离心8分钟后,在血清管中从自体血液制备PRF凝块。在PRF-Box®中将PRF凝块加工成PRF膜。将PRF膜缝合到角膜溃疡床上。每只狗在手术后第5-7、12-14和30-40天进行随访。还进行了最后的长期随访。
结果：在七只狗中的六只中观察到愈合和“良好”质量的PRF膜的阳性结果。一只狗的纤维蛋白原水平低于正常范围，PRF膜质量“差”。这只狗在手术后13天出现了后代囊肿，需要进行抢救手术。所有狗的平均愈合时间为9±5.5天。疤痕最小,角膜色素沉着,在手术后288±44天的最终长期随访中观察到血管形成。
结论：PRF膜作为角膜溃疡重建手术的移植材料是成功的。低纤维蛋白原似乎对PRF膜的质量有负面影响，显示外科医生在手术前评估PRF膜质量的重要性。
方法：7只患有复杂角膜溃疡的狗。
方法：完整的眼科检查，血液学,术前进行纤维蛋白原分析。在水平Bio-PRF®离心机中以700xg离心8分钟后,在血清管中从自体血液制备PRF凝块。在PRF-Box®中将PRF凝块加工成PRF膜。将PRF膜缝合到角膜溃疡床上。每只狗在手术后第5-7、12-14和30-40天进行随访。还进行了最后的长期随访。
结果：在七只狗中的六只中观察到愈合和“良好”质量的PRF膜的阳性结果。一只狗的纤维蛋白原水平低于正常范围，PRF膜质量“差”。这只狗在手术后13天出现了后代囊肿，需要进行抢救手术。所有狗的平均愈合时间为9±5.5天。疤痕最小,角膜色素沉着,在手术后288±44天的最终长期随访中观察到血管形成。
结论：PRF膜作为角膜溃疡重建手术的移植材料是成功的。低纤维蛋白原似乎对PRF膜的质量有负面影响，显示外科医生在手术前评估PRF膜质量的重要性。
