PDF(Pubmed)
Abstract：
Bone immune responses based on macrophages are critical in the osteogenesis of bone abnormalities. In general, M2 macrophage facilitate the promotion of osteogenesis, as well, M1 macrophage play an important role in early bone healing, as confirmed by previous studies. However, it is not clear how M1 macrophage are involved in the bone immune response. MiR-21a-5p is a highly expressed microRNA in M1 macrophage in contrast to M2. Therefore, the current work sought to ascertain the influence of M1 macrophage on bone healing via exosomal miR-21a-5p and the probable mechanism. We discovered that injecting M1 macrophage exosomes overexpressing miR-21a-5p into bone defect locations enhanced bone regeneration in vivo. Furthermore, by directly targeting GATA2, miR-21a-5p accelerated MC3T3-E1 osteogenic differentiation. Our findings showed that exosomal miR-21a-5p from M1 macrophage may be transported to osteoblasts and target GATA2 to enhance bone defect healing.
摘要：
基于巨噬细胞的骨免疫应答在骨异常的成骨中至关重要。总的来说,M2巨噬细胞促进成骨,还有,M1巨噬细胞在骨早期愈合中起重要作用，正如以前的研究所证实的那样。然而,尚不清楚M1巨噬细胞如何参与骨骼免疫反应。与M2相比,MiR-21a-5p是M1巨噬细胞中高度表达的微小RNA。因此,目前的工作试图确定M1巨噬细胞通过外泌体miR-21a-5p对骨愈合的影响及其可能的机制。我们发现，将过表达miR-21a-5p的M1巨噬细胞外泌体注射到骨缺损位置可增强体内骨再生。此外，通过直接靶向GATA2，miR-21a-5p加速MC3T3-E1成骨分化。我们的发现表明，来自M1巨噬细胞的外泌体miR-21a-5p可能被转运到成骨细胞并靶向GATA2以促进骨缺损愈合。
