UNASSIGNED: To improve salinity tolerance and investigate the effects of choline on the physiological and lipidomic responses of turfgrass plants under salinity stress conditions, exogenous choline chloride was applied to Kentucky bluegrass exposed to salt stress.
UNASSIGNED: From physiological indicators, exogenous choline chloride could alleviate salt stress injury in Kentucky bluegrass. Lipid analysis showed that exogenous choline chloride under salt-stress conditions remodeled the content of phospholipids, glycolipids, and lysophospholipids. Monogalactosyl diacylglycerol, digalactosyl diacylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, and lysophosphatidylcholine content were increased and phosphatidic acid content were decreased in plants after exogenous choline chloride under salt treatment. Plant leaf choline content increased, but GB was not detected in exogenous choline chloride treatment plants under nonstress or salt-stress conditions.
UNASSIGNED: GB synthesis pathway related genes showed no clear change to choline chloride treatment, whereas cytidyldiphosphate-choline (CDP-choline) pathway genes were upregulated by choline chloride treatment. These results reveal that lipid remodeling through choline metabolism plays an important role in the salt tolerance mechanism of Kentucky bluegrass. Furthermore, the lipids selected in this study could serve as biomarkers for further improvement of salt-sensitive grass species.
■为了提高耐盐性,并研究胆碱对盐分胁迫条件下草坪草植物的生理和脂质响应的影响,将外源氯化胆碱应用于盐胁迫下的肯塔基蓝草。
■从生理指标来看,外源氯化胆碱可以减轻草地早熟禾的盐胁迫伤害。脂质分析表明,盐胁迫条件下外源氯化胆碱改变了磷脂的含量,糖脂,和溶血磷脂.单半乳糖基二酰基甘油,二半乳糖基二酰基甘油,磷脂酰胆碱,磷脂酰乙醇胺,磷脂酰丝氨酸,外源氯化胆碱盐处理后,植物中溶血磷脂酰胆碱含量增加,磷脂酸含量降低。植物叶片胆碱含量增加,但是在非胁迫或盐胁迫条件下,外源氯化胆碱处理植物中未检测到GB。
■GB合成途径相关基因对氯化胆碱处理没有明显变化,而磷脂酰二磷酸胆碱(CDP-胆碱)途径基因被氯化胆碱处理上调。这些结果表明,通过胆碱代谢进行的脂质重塑在草地早熟禾的耐盐机制中起着重要作用。此外,本研究中选择的脂质可作为进一步改善盐敏感性草种的生物标志物。