Abstract:
Sulfamethazine (SM2) is an antibacterial drug,which has been extensively used in human and veterinary medicine, long-term consumption of which may lead to the accumulation of sulfonamides in the body. Detection of sulfonamides often uses microbiological approaches, mass spectrometry and chromatography, which are expensive and time-consuming. Surface-enhanced Raman scattering-based immunochromatographic assay (SERS-ICA) has been recently applied in the detection. Herein, a Janus-labeled Au nanoparticle with subnanosized SiO2-monoclonal antibody and SERS reporter (DTNB) modified simultaneously (mAbAuNpDTNB) has been developed in a SERS-based lateral flow immunosensor, which can be used for rapid, quantitative and ultrasensitive detection of sulfamethazine residue in milk. The mAbAuNpDTNB exhibits a specific array on a paper stripe, which not only identifies sulfamethazine but also straightforwardly exposes the Raman reporter between the AuNps via self-assembly. The detection sensitivity of SERS-ICA for sulfamethazine reached 0.1 pg/mL, which was far below the previously published value by ELISA and the maximum residue limit set by the European Union. The entire SERS-ICA detection for sulfamethazine was completed within 15 min. Furthermore, high accuracy for this assay was exhibited in the spiking experiment with a recovery percentage of 88.1%-112.7%. The results demonstrated that this SERS-ICA can potentially be applied in point-of-care testing as an ultrasensitive and quantitative to semi-quantitative analytical method.
摘要:
磺胺二甲嘧啶(SM2)是一种抗菌药物,已广泛应用于人类和兽医学。长期食用可能导致磺胺类药物在体内积累。磺胺类药物的检测通常使用微生物学方法,质谱和色谱,这是昂贵和耗时的。基于表面增强拉曼散射的免疫色谱分析(SERS-ICA)最近已被用于检测。在这里,已在基于SERS的侧流免疫传感器中开发了具有亚纳米尺寸的SiO2单克隆抗体和SERS报道分子(DTNB)同时修饰的Janus标记的Au纳米颗粒(mAbAuNpDTNB),可以用于快速,牛奶中磺胺二甲嘧啶残留量的定量和超灵敏检测。mAbAuNpDTNB在纸条纹上展示了一个特定的阵列,它不仅可以识别磺胺二甲嘧啶,而且可以通过自组装直接暴露AuNps之间的拉曼报道分子。SERS-ICA对磺胺二甲嘧啶的检测灵敏度达到0.1pg/mL,远低于先前通过ELISA公布的值和欧盟设定的最大残留限量。磺胺二甲嘧啶的整个SERS-ICA检测在15分钟内完成。此外,在加标实验中,该测定具有很高的准确性,回收率为88.1%-112.7%。结果表明,这种SERS-ICA可以作为超灵敏和定量到半定量分析方法应用于即时测试。