Abstract：
Multiplexing in biological assays allows the simultaneous detection of multiple analytes in a single reaction, which reduces time, labor, and cost as compared to single reaction-based detection methods. Microsphere- or bead-based suspension array technologies, such as the Luminex® xMAP® system, offer high-throughput detection of nucleic acids through a variety of different assay chemistries. Common with most nucleic acid chemistries, for bead-based or other microarray technologies, is the need for efficient extraction and purification of the nucleic acids from the specimen of interest. Often, the optimal method will be dictated by the requirements of the up-front enzymatic chemistry, such as PCR, primer extension, branched DNA (bDNA), etc. For bead-based microarray platforms, the user must also be cognizant of proteins and other contaminants present in reactions that require heat denaturation, as that can lead to bead aggregation or agglutination, preventing the reading of assay results. This review describes and highlights some of the nucleic acid extraction and purification methods that have been used successfully for bead-based nucleic acid analysis, for both prokaryotic and eucaryotic nucleic acids, from a variety of sample types.
摘要：
生物测定中的多路复用允许在单个反应中同时检测多种分析物，这减少了时间，劳动,与基于单一反应的检测方法相比，成本也很高。基于微球或珠子的悬浮阵列技术，例如Luminex®xMAP®系统，通过各种不同的分析化学提供核酸的高通量检测。常见的大多数核酸化学，对于基于珠子或其他微阵列技术，需要从感兴趣的样本中有效提取和纯化核酸。通常,最佳方法将由前期酶化学的要求决定，如PCR，引物延伸，分支DNA（bDNA），等。对于基于珠子的微阵列平台，用户还必须认识到需要热变性的反应中存在的蛋白质和其他污染物，因为这可能导致珠子聚集或凝集，防止读取测定结果。这篇综述描述并强调了一些已成功用于基于珠子的核酸分析的核酸提取和纯化方法，对于原核和真核核酸，从各种样品类型。
