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Abstract：
UNASSIGNED: Glioblastoma (Gb) is the most common primary malignant tumor of brain with poor prognosis. Immune cells are the main factors affecting the prognosis of Gb, tumor-associated macrophages (TAMs) are the predominant infiltrating immune cell population in the immune microenvironment of Gb. Analyzing the relationship between magnetic resonance imaging (MRI) features and TAMs of Gb, and using imaging features to characterize the infiltration level of TAMs in tumor tissue may provide indicators for clinical decision-making and prognosis evaluation of Gb.
UNASSIGNED: Data from 140 in patients with isocitrate dehydrogenase (IDH) wild-type Gb diagnosed via histopathology and molecular diagnosis in the Second Hospital of Lanzhou University from January 2018 to April 2022 were collected in this retrospective, cross-sectional study. MRI images were reviewed for lesion location, cyst, necrosis, hemorrhage, contrast-enhanced T1-weighted MRI signal intensity, average apparent diffusion coefficient (ADCmean), and minimum apparent diffusion coefficient (ADCmin). Immunohistochemical staining with anti-CD163 and anti-CD68 antibodies was employed for macrophage detection. The positive cell percentage was estimated in 9 microscopic fields at 400× magnification per whole-slide image with ImageJ software (National Institutes of Health). Additionally, the relationship between MRI features, molecular, states and the positive CD68 and CD163 expression was analyzed.
UNASSIGNED: Our study discovered that the mean or median values of CD68+ and CD163+ TAMs were 7.39% and 14.98%, respectively. There was an obvious correlation between CD163+ TAMs and CD68+ TAMs (r=0.497; P=0.000). CD68+ and CD163+ macrophage infiltration correlated with age at diagnosis in patients with Gb (CD68+: r=0.230, P=0.006; CD163+: r=0.172, P=0.042). The levels of Gb TAM infiltration in different tumor locations varied, with the temporal lobe having the highest CD163+ macrophage and CD68+ macrophage infiltration (18.58% and 9.46%, respectively). CD163+ macrophage infiltration was positively correlated with ADCmean (r=0.208; P=0.014). The infiltration of CD68+ macrophages differed significantly between groups with varying degrees of tumor enhancement (H =4.228; P=0.017). There was a significant difference in CD68+ TAMs and CD163+ TAMs between the wild-type and mutant-type telomerase reverse transcriptase (TERT) types (P=0.004 and P=0.031, respectively).
UNASSIGNED: Age, location of the tumor, degree of tumor enhancement, ADC value, and TERT mutation status were associated with macrophage infiltration. These findings may serve as an effective tool for characterizing the tumor microenvironment in patients with Gb.
UNASSIGNED: Data from 140 in patients with isocitrate dehydrogenase (IDH) wild-type Gb diagnosed via histopathology and molecular diagnosis in the Second Hospital of Lanzhou University from January 2018 to April 2022 were collected in this retrospective, cross-sectional study. MRI images were reviewed for lesion location, cyst, necrosis, hemorrhage, contrast-enhanced T1-weighted MRI signal intensity, average apparent diffusion coefficient (ADCmean), and minimum apparent diffusion coefficient (ADCmin). Immunohistochemical staining with anti-CD163 and anti-CD68 antibodies was employed for macrophage detection. The positive cell percentage was estimated in 9 microscopic fields at 400× magnification per whole-slide image with ImageJ software (National Institutes of Health). Additionally, the relationship between MRI features, molecular, states and the positive CD68 and CD163 expression was analyzed.
UNASSIGNED: Our study discovered that the mean or median values of CD68+ and CD163+ TAMs were 7.39% and 14.98%, respectively. There was an obvious correlation between CD163+ TAMs and CD68+ TAMs (r=0.497; P=0.000). CD68+ and CD163+ macrophage infiltration correlated with age at diagnosis in patients with Gb (CD68+: r=0.230, P=0.006; CD163+: r=0.172, P=0.042). The levels of Gb TAM infiltration in different tumor locations varied, with the temporal lobe having the highest CD163+ macrophage and CD68+ macrophage infiltration (18.58% and 9.46%, respectively). CD163+ macrophage infiltration was positively correlated with ADCmean (r=0.208; P=0.014). The infiltration of CD68+ macrophages differed significantly between groups with varying degrees of tumor enhancement (H =4.228; P=0.017). There was a significant difference in CD68+ TAMs and CD163+ TAMs between the wild-type and mutant-type telomerase reverse transcriptase (TERT) types (P=0.004 and P=0.031, respectively).
UNASSIGNED: Age, location of the tumor, degree of tumor enhancement, ADC value, and TERT mutation status were associated with macrophage infiltration. These findings may serve as an effective tool for characterizing the tumor microenvironment in patients with Gb.
摘要：
■胶质母细胞瘤（Gb）是最常见的原发性脑恶性肿瘤，预后较差。免疫细胞是影响Gb预后的主要因素,肿瘤相关巨噬细胞(TAMs)是Gb免疫微环境中主要的浸润免疫细胞群。分析磁共振成像(MRI)特征与Gb、利用影像学特征表征肿瘤组织中TAMs的浸润程度，可为临床决策和预后评估提供指标。
■收集2018年1月至2022年4月在兰州大学第二医院通过组织病理学和分子诊断诊断的140例异柠檬酸脱氢酶（IDH）野生型Gb患者的数据。横断面研究。MRI图像检查病灶位置，囊肿,坏死,出血,对比增强T1加权MRI信号强度,平均表观扩散系数(ADCmean)，和最小表观扩散系数(ADCmin)。使用抗CD163和抗CD68抗体的免疫组织化学染色用于巨噬细胞检测。用ImageJ软件(美国国立卫生研究院)在9个显微镜视野中以400倍放大每全载玻片图像估计阳性细胞百分比。此外,MRI特征之间的关系，分子，状态，并分析CD68和CD163的阳性表达。
■我们的研究发现，CD68+和CD163+TAM的平均值或中值分别为7.39%和14.98%，分别。CD163+TAMs与CD68+TAMs有明显的相关性(r=0.497;P=0.000)。Gb患者CD68+和CD163+巨噬细胞浸润与诊断年龄相关(CD68+:r=0.230,P=0.006;CD163+:r=0.172,P=0.042)。GbTAM在不同肿瘤部位的浸润程度不同,颞叶CD163+巨噬细胞和CD68+巨噬细胞浸润最高(18.58%和9.46%,分别)。CD163+巨噬细胞浸润与ADCmean呈正相关(r=0.208;P=0.014)。CD68+巨噬细胞浸润在不同程度的肿瘤增强组间差异显著(H=4.228;P=0.017)。野生型和突变型端粒酶逆转录酶（TERT）类型之间的CD68TAM和CD163TAM存在显着差异（分别为P=0.004和P=0.031）。
■年龄，肿瘤的位置，肿瘤增强程度，ADC值,TERT突变状态与巨噬细胞浸润相关。这些发现可能是表征Gb患者肿瘤微环境的有效工具。
■收集2018年1月至2022年4月在兰州大学第二医院通过组织病理学和分子诊断诊断的140例异柠檬酸脱氢酶（IDH）野生型Gb患者的数据。横断面研究。MRI图像检查病灶位置，囊肿,坏死,出血,对比增强T1加权MRI信号强度,平均表观扩散系数(ADCmean)，和最小表观扩散系数(ADCmin)。使用抗CD163和抗CD68抗体的免疫组织化学染色用于巨噬细胞检测。用ImageJ软件(美国国立卫生研究院)在9个显微镜视野中以400倍放大每全载玻片图像估计阳性细胞百分比。此外,MRI特征之间的关系，分子，状态，并分析CD68和CD163的阳性表达。
■我们的研究发现，CD68+和CD163+TAM的平均值或中值分别为7.39%和14.98%，分别。CD163+TAMs与CD68+TAMs有明显的相关性(r=0.497;P=0.000)。Gb患者CD68+和CD163+巨噬细胞浸润与诊断年龄相关(CD68+:r=0.230,P=0.006;CD163+:r=0.172,P=0.042)。GbTAM在不同肿瘤部位的浸润程度不同,颞叶CD163+巨噬细胞和CD68+巨噬细胞浸润最高(18.58%和9.46%,分别)。CD163+巨噬细胞浸润与ADCmean呈正相关(r=0.208;P=0.014)。CD68+巨噬细胞浸润在不同程度的肿瘤增强组间差异显著(H=4.228;P=0.017)。野生型和突变型端粒酶逆转录酶（TERT）类型之间的CD68TAM和CD163TAM存在显着差异（分别为P=0.004和P=0.031）。
■年龄，肿瘤的位置，肿瘤增强程度，ADC值,TERT突变状态与巨噬细胞浸润相关。这些发现可能是表征Gb患者肿瘤微环境的有效工具。
