PDF(Pubmed)
Abstract：
The spheroid bacterium Staphylococcus aureus is often used as a model of morphogenesis due to its apparently simple cell cycle. S. aureus has many cell division proteins that are conserved across bacteria alluding to common functions. However, despite intensive study, we still do not know the roles of many of these components. Here, we have examined the functions of the paralogues DivIVA and GpsB in the S. aureus cell cycle. Cells lacking gpsB display a more spherical phenotype than the wild-type cells, which is associated with a decrease in peripheral cell wall peptidoglycan synthesis. This correlates with increased localization of penicillin-binding proteins at the developing septum, notably PBPs 2 and 3. Our results highlight the role of GpsB as an apparent regulator of cell morphogenesis in S. aureus.
摘要：
球形细菌金黄色葡萄球菌由于其明显简单的细胞周期而经常用作形态发生的模型。金黄色葡萄球菌具有许多细胞分裂蛋白，它们在细菌中保守，暗示了共同的功能。然而,尽管深入研究,我们仍然不知道其中许多组件的作用。这里,我们已经检查了副同源物DivIVA和GpsB在金黄色葡萄球菌细胞周期中的功能。缺乏gpsB的细胞比野生型细胞表现出更球形的表型，这与外周细胞壁肽聚糖合成的减少有关。这与青霉素结合蛋白在发育中隔的定位增加有关，特别是PBPs2和3。我们的结果强调了GpsB作为金黄色葡萄球菌细胞形态发生的明显调节因子的作用。
