Abstract：
NITRATE TRANSPORTER 1 (NRT1)/PEPTIDETRANSPORTER (PTR) family (NPF) plays a significant role in nitrate transport. However, little is known about the NPF genes in sweet cherry. In this study, a total of 60 PaNPF genes in sweet cherry were identified by bioinformatics, which were divided into 8 families. Transcriptomic analysis showed that most PaNPF genes responded to both low and high nitrate conditions, especially PaNPF5.5, which was highly up-regulated under high nitrate condition. Molecular analysis showed that PaNPF5.5 was a transporter localized to the cell membrane. Further functional studies found that PaNPF5.5 overexpression promoted the growth of sweet cherry rootstock Gisela 6 by accelerating the nitrogen absorption process under high nitrate environment. Taken together, we believe that PaNPF5.5 plays an important role in regulating the transport of nitrate at high nitrate conditions, and provides a promising method for improving nitrate absorption efficiency at nitrogen excess environment.
摘要：
硝酸盐转运蛋白1(NRT1)/肽转运蛋白(PTR)家族(NPF)在硝酸盐转运中起重要作用。然而,对甜樱桃中的NPF基因知之甚少。在这项研究中,通过生物信息学方法鉴定了甜樱桃中的60个PaNPF基因，分为8个家庭。转录组分析表明，大多数PaNPF基因对低硝酸盐和高硝酸盐条件都有反应，特别是PaNPF5.5，在高硝酸盐条件下高度上调。分子分析表明，PaNPF5.5是位于细胞膜上的转运蛋白。进一步的功能研究发现，在高硝酸盐环境下，PaNPF5.5过表达通过加速氮吸收过程促进甜樱桃砧木Gisela6的生长。一起来看,我们认为，在高硝酸盐条件下，PaNPF5.5在调节硝酸盐的运输中起着重要作用，并提供了一种在氮过量环境下提高硝酸盐吸收效率的有前途的方法。
