Abstract：
An accurate species delimitation is critical for biological studies. In this context, the use of molecular techniques along with species delimitation methods would help to a rapid and accurate biodiversity assessment. The species delimitation methods cluster data sets of orthologous sequences in molecular operational taxonomic units (MOTU). In particular, the methods based on a single gene are easily integrated with the widely used DNA barcoding approach. We developed SPdel a user-friendly pipeline to integrate different single-gene species delimitation methods. SPdel is designed to calculate and compare MOTUs obtained by different species delimitation approaches. SPdel also outputs diverse ready-to-publish quality figures, that facilitate the interpretation of results. SPdel aims to help researchers use species delimitation methods that would improve biodiversity studies.
摘要：
准确的物种定界对于生物学研究至关重要。在这种情况下，分子技术和物种划界方法的使用将有助于快速和准确的生物多样性评估。物种定界方法对分子操作分类单位（MOTU）中的直系同源序列数据集进行聚类。特别是,基于单个基因的方法很容易与广泛使用的DNA条形码方法相结合。我们开发了SPdel一个用户友好的管道来整合不同的单基因物种定界方法。SPdel旨在计算和比较通过不同物种划界方法获得的MOTU。SPdel还输出各种准备发布的质量数据，这有助于解释结果。SPdel旨在帮助研究人员使用物种划界方法来改善生物多样性研究。
