PDF(Pubmed)
Abstract：
UNASSIGNED: Correlations between posttranslational modifications and atrial fibrillation (AF) have been demonstrated in recent studies. However, it is still unclear whether and how ubiquitylated proteins relate to AF in the left atrial appendage of patients with AF and valvular heart disease.
UNASSIGNED: Through LC-MS/MS analyses, we performed a study on tissues from eighteen subjects (9 with sinus rhythm and 9 with AF) who underwent cardiac valvular surgery. Specifically, we explored the ubiquitination profiles of left atrial appendage samples.
UNASSIGNED: In summary, after the quantification ratios for the upregulated and downregulated ubiquitination cutoff values were set at >1.5 and <1:1.5, respectively, a total of 271 sites in 162 proteins exhibiting upregulated ubiquitination and 467 sites in 156 proteins exhibiting downregulated ubiquitination were identified. The ubiquitylated proteins in the AF samples were enriched in proteins associated with ribosomes, hypertrophic cardiomyopathy (HCM), glycolysis, and endocytosis.
UNASSIGNED: Our findings can be used to clarify differences in the ubiquitination levels of ribosome-related and HCM-related proteins, especially titin (TTN) and myosin heavy chain 6 (MYH6), in patients with AF, and therefore, regulating ubiquitination may be a feasible strategy for AF.
UNASSIGNED: Through LC-MS/MS analyses, we performed a study on tissues from eighteen subjects (9 with sinus rhythm and 9 with AF) who underwent cardiac valvular surgery. Specifically, we explored the ubiquitination profiles of left atrial appendage samples.
UNASSIGNED: In summary, after the quantification ratios for the upregulated and downregulated ubiquitination cutoff values were set at >1.5 and <1:1.5, respectively, a total of 271 sites in 162 proteins exhibiting upregulated ubiquitination and 467 sites in 156 proteins exhibiting downregulated ubiquitination were identified. The ubiquitylated proteins in the AF samples were enriched in proteins associated with ribosomes, hypertrophic cardiomyopathy (HCM), glycolysis, and endocytosis.
UNASSIGNED: Our findings can be used to clarify differences in the ubiquitination levels of ribosome-related and HCM-related proteins, especially titin (TTN) and myosin heavy chain 6 (MYH6), in patients with AF, and therefore, regulating ubiquitination may be a feasible strategy for AF.
摘要：
■翻译后修饰与心房颤动（AF）之间的相关性已在最近的研究中得到证实。然而,目前尚不清楚泛素化蛋白是否以及如何与房颤和瓣膜性心脏病患者左心耳中的房颤相关.
■通过LC-MS/MS分析，我们对18例心脏瓣膜手术患者(9例窦性心律患者和9例房颤患者)的组织进行了研究.具体来说,我们研究了左心耳样本的泛素化特征.
■总之，在上调和下调的泛素化截止值的定量比率分别设定为>1.5和<1:1.5后，在162种表现出上调的泛素化的蛋白质中总共有271个位点,在156种表现出下调的泛素化的蛋白质中总共有467个位点.AF样品中的泛素化蛋白富含与核糖体相关的蛋白，肥厚型心肌病（HCM），糖酵解,和内吞作用。
■我们的发现可用于阐明核糖体相关和HCM相关蛋白的泛素化水平的差异，特别是肌动蛋白（TTN）和肌球蛋白重链6（MYH6），在房颤患者中，因此,调节泛素化可能是房颤的可行策略。
■通过LC-MS/MS分析，我们对18例心脏瓣膜手术患者(9例窦性心律患者和9例房颤患者)的组织进行了研究.具体来说,我们研究了左心耳样本的泛素化特征.
■总之，在上调和下调的泛素化截止值的定量比率分别设定为>1.5和<1:1.5后，在162种表现出上调的泛素化的蛋白质中总共有271个位点,在156种表现出下调的泛素化的蛋白质中总共有467个位点.AF样品中的泛素化蛋白富含与核糖体相关的蛋白，肥厚型心肌病（HCM），糖酵解,和内吞作用。
■我们的发现可用于阐明核糖体相关和HCM相关蛋白的泛素化水平的差异，特别是肌动蛋白（TTN）和肌球蛋白重链6（MYH6），在房颤患者中，因此,调节泛素化可能是房颤的可行策略。
