PDF(Pubmed)
Abstract：
Dental calculus is becoming a crucial material in the study of past populations with increasing interest in its proteomic and genomic content. Here, we suggest further development of a protocol for analysis of ancient proteins and a combined approach for subsequent ancient DNA extraction. We tested the protocol on recent teeth, and the optimized protocol was applied to ancient tooth to limit the destruction of calculus as it is a precious and irreplaceable source of dietary, microbiological, and ecological information in the archeological context. Finally, the applicability of the protocol was demonstrated on samples of the ancient calculus.
摘要：
牙结石正在成为研究过去人群的重要材料，对其蛋白质组学和基因组含量的兴趣日益浓厚。这里,我们建议进一步开发一种用于分析古代蛋白质的协议，并为随后的古代DNA提取提供一种组合方法。我们在最近的牙齿上测试了协议，并将优化的方案应用于古老的牙齿，以限制牙结石的破坏，因为它是一种珍贵且不可替代的饮食来源，微生物,和考古背景下的生态信息。最后,该方案的适用性在古代微积分的样本上得到了证明。
