Abstract：
We have shown in the past decade, for the first time in a bivalve mollusc, detection, isolation, and purification of β-1,3 glucan binding protein (β-GBP) in the plasma of the marine mussel Perna viridis and demonstrated its role in a nonself-induced activation of plasma prophenoloxidase system. In this study, we present evidence for its ability to function as an opsonin during phagocytosis of trypsinized yeast cells by the hemocytes of P. viridis. The in vitro pretreatment of target cells (trypsinized yeast cells) with β-GBP enhanced the phagocytic response of hemocytes. Such β-GBP-mediated enhanced phagocytic response appeared to be dose dependent. This opsono-phagocytic response could be inhibited by the presence of laminarin (a polymer of β-1,3 glucans), glucose, as well as polyclonal antibodies raised against β-GBP. These observations clearly indicate that the plasma β-GBP can possibly recognize and bind to β-1,3 glucans on the surface of targets and facilitate hemocyte recognition processes possibly by forming a bridge between the hemocytes and the target, consequently leading to opsono-phagocytosis. These observations together with our earlier annotations indicate the multifunctional potential of plasma β-GBP in the marine mussel P. viridis.
摘要：
在过去的十年中，我们已经证明，第一次在双壳软体动物身上，检测,隔离,并纯化了海洋贻贝Pernaviridis血浆中的β-1,3葡聚糖结合蛋白（β-GBP），并证明了其在非自诱导的血浆酚氧化酶原系统激活中的作用。在这项研究中,我们提供了证据，证明其在通过绿脓杆菌的血细胞吞噬胰蛋白酶化的酵母细胞过程中具有调理素的功能。用β-GBP体外预处理靶细胞（胰蛋白酶化酵母细胞）可增强血细胞的吞噬反应。这种β-GBP介导的增强的吞噬反应似乎是剂量依赖性的。这种调理吞噬反应可以被存在的海带多糖（β-1,3葡聚糖的聚合物）抑制，葡萄糖，以及抗β-GBP的多克隆抗体。这些观察结果清楚地表明，血浆β-GBP可能识别并结合目标表面的β-1,3葡聚糖，并可能通过在血细胞和目标之间形成桥梁来促进血细胞识别过程。因此导致opsono吞噬作用。这些观察结果以及我们先前的注释表明，血浆β-GBP在海洋贻贝中的多功能潜力。
