PDF(Pubmed)
Abstract：
UNASSIGNED: Circular RNAs (circRNAs) demonstrated critical roles within developing tumors and treatment resistance in an increasing body of research. The aim was to look into the functions and processes of hsa_circ_0003489 in the non-small cell lung cancer (NSCLC) paclitaxel (PTX) resistance.
UNASSIGNED: NSCLC cell-based cultures including A549 and H460 were employed for such an investigation. hsa_circ_0003489, miR-98-5p, and insulin-like growth factor 2 (IGF2) expression-profiles were evaluated with a quantitative real-time polymerase chain reaction (RT-qPCR). The PTX resistance was determined using MTT assay, and the ELISA test measured IGF2 expression. Facilitating corroboration for miR-98-5p relation and hsa_circ_0003489 or IGF2, a dual-luciferase reporter method was applied.
UNASSIGNED: The hsa_circ_0003489 level was raised in cells and tissues from PTX-resistant (PR) NSCLC. In PR NSCLC cells, hsa_circ_0003489 knockdown reduced PTX resistance. For the purpose of the mechanism study, hsa_circ_0003489 knockdown substantially reduced IGF2 expression via miR-98-5p sponging, improving PTX sensitivity in PR NSCLC.
UNASSIGNED: Through miR-98-5p/IGF2 axis control, hsa_circ_0003489 knockdown helped NSCLC overcome PTX resistance, suggesting a potential circRNA-targeted therapy for the disease.
UNASSIGNED: NSCLC cell-based cultures including A549 and H460 were employed for such an investigation. hsa_circ_0003489, miR-98-5p, and insulin-like growth factor 2 (IGF2) expression-profiles were evaluated with a quantitative real-time polymerase chain reaction (RT-qPCR). The PTX resistance was determined using MTT assay, and the ELISA test measured IGF2 expression. Facilitating corroboration for miR-98-5p relation and hsa_circ_0003489 or IGF2, a dual-luciferase reporter method was applied.
UNASSIGNED: The hsa_circ_0003489 level was raised in cells and tissues from PTX-resistant (PR) NSCLC. In PR NSCLC cells, hsa_circ_0003489 knockdown reduced PTX resistance. For the purpose of the mechanism study, hsa_circ_0003489 knockdown substantially reduced IGF2 expression via miR-98-5p sponging, improving PTX sensitivity in PR NSCLC.
UNASSIGNED: Through miR-98-5p/IGF2 axis control, hsa_circ_0003489 knockdown helped NSCLC overcome PTX resistance, suggesting a potential circRNA-targeted therapy for the disease.
摘要：
■环状RNA（circularRNAs，circRNAs）在越来越多的研究中证明了在发展肿瘤和治疗抗性中的关键作用。目的是研究hsa_circ_0003489在非小细胞肺癌（NSCLC）紫杉醇（PTX）耐药中的功能和过程。
包括A549和H460的基于NSCLC细胞的培养物用于这样的研究。hsa_circ_0003489,miR-98-5p,用定量实时聚合酶链反应（RT-qPCR）评估胰岛素样生长因子2（IGF2）的表达谱。使用MTT测定法测定PTX抗性,和ELISA测试测量IGF2表达。促进miR-98-5p关系和hsa_circ_0003489或IGF2的确证,应用双荧光素酶报告方法。
■来自PTX抗性(PR)NSCLC的细胞和组织中的hsa_circ_0003489水平升高。在PRNSCLC细胞中，hsa_circ_0003489敲低降低了PTX抗性。出于机理研究的目的，hsa_circ_0003489敲低通过miR-98-5p海绵作用显著降低IGF2表达，改善PTX在PR非小细胞肺癌中的敏感性。
■通过miR-98-5p/IGF2轴控制，hsa_circ_0003489敲除帮助非小细胞肺癌克服PTX耐药，提示该疾病的潜在circRNA靶向治疗。
包括A549和H460的基于NSCLC细胞的培养物用于这样的研究。hsa_circ_0003489,miR-98-5p,用定量实时聚合酶链反应（RT-qPCR）评估胰岛素样生长因子2（IGF2）的表达谱。使用MTT测定法测定PTX抗性,和ELISA测试测量IGF2表达。促进miR-98-5p关系和hsa_circ_0003489或IGF2的确证,应用双荧光素酶报告方法。
■来自PTX抗性(PR)NSCLC的细胞和组织中的hsa_circ_0003489水平升高。在PRNSCLC细胞中，hsa_circ_0003489敲低降低了PTX抗性。出于机理研究的目的，hsa_circ_0003489敲低通过miR-98-5p海绵作用显著降低IGF2表达，改善PTX在PR非小细胞肺癌中的敏感性。
■通过miR-98-5p/IGF2轴控制，hsa_circ_0003489敲除帮助非小细胞肺癌克服PTX耐药，提示该疾病的潜在circRNA靶向治疗。
