PDF(Pubmed)
Abstract：
UNASSIGNED: The blood-feeding behavior of kissing bugs (subfamily Triatominae, family Reduviidae, order Hemiptera) means they are potential vectors of multiple humans pathogens. However, investigations of vector-borne pathogens harbored by kissing bugs are rare.
UNASSIGNED: In the current study, 22 adult kissing bugs (Triatoma rubrofasciata) were captured in Huizhou City, Guangdong Province, south China. The presence of vector-borne pathogens in the kissing bugs was tested, and the genetic diversity of these potential pathogens was investigated.
UNASSIGNED: All the kissing bugs were negative for Anaplasmataceae bacteria, Rickettsia, and Coxiella. Bartonella DNA was detected in 36.4% (8/22) of the kissing bugs. The sequences of the Bartonella gltA genes divided into two clades in a phylogenetic tree, with close relationships to B. tribocorum and uncultured Bartonella sp. clone MYR-283, respectively. All the groEL sequences were closely related to those of B. kosoyi (identity 98.75%-100%). The ftsZ and rpoB sequences were most closely related to those of B. elizabethae, a recognized human pathogen, with nucleotide similarities of 98.70%-100% and 99.45%-100%, respectively.
UNASSIGNED: We report the detection of Bartonella DNA in Triatoma kissing bugs in southern China. Although the sample size is limited, the high positive rate of detection of Bartonella DNA, the close relationship of the gene sequences to those of zoonotic Bartonella species, and the distribution of the kissing bugs near human residences, hint at a risk to public health.
UNASSIGNED: In the current study, 22 adult kissing bugs (Triatoma rubrofasciata) were captured in Huizhou City, Guangdong Province, south China. The presence of vector-borne pathogens in the kissing bugs was tested, and the genetic diversity of these potential pathogens was investigated.
UNASSIGNED: All the kissing bugs were negative for Anaplasmataceae bacteria, Rickettsia, and Coxiella. Bartonella DNA was detected in 36.4% (8/22) of the kissing bugs. The sequences of the Bartonella gltA genes divided into two clades in a phylogenetic tree, with close relationships to B. tribocorum and uncultured Bartonella sp. clone MYR-283, respectively. All the groEL sequences were closely related to those of B. kosoyi (identity 98.75%-100%). The ftsZ and rpoB sequences were most closely related to those of B. elizabethae, a recognized human pathogen, with nucleotide similarities of 98.70%-100% and 99.45%-100%, respectively.
UNASSIGNED: We report the detection of Bartonella DNA in Triatoma kissing bugs in southern China. Although the sample size is limited, the high positive rate of detection of Bartonella DNA, the close relationship of the gene sequences to those of zoonotic Bartonella species, and the distribution of the kissing bugs near human residences, hint at a risk to public health.
摘要：
■接吻虫子的采血行为（Triatominae亚科，科Reduviidae,半翅目）意味着它们是多种人类病原体的潜在载体。然而,对接吻虫子所携带的媒介传播病原体的调查很少。
■在当前的研究中，在惠州市捕获了22只成年接吻虫（Triatomarubrofasciata），广东省,中国南方。测试了接吻虫子中媒介传播的病原体的存在，并研究了这些潜在病原体的遗传多样性。
■所有的接吻虫子都是无性菌科细菌,立克次体,和柯西拉。在36.4%(8/22)的接吻虫中检测到巴尔通体DNA。巴尔通氏菌gltA基因的序列在系统发育树中分为两个分支，与B.tribocorum和未培养的Bartonellasp。克隆MYR-283。所有groEL序列均与B.kosoyi的序列密切相关（同一性98.75％-100％）。ftsZ和rpoB序列与elizabethae的序列关系最密切，一种公认的人类病原体，核苷酸相似性为98.70%-100%和99.45%-100%，分别。
■我们报告了在中国南方的Triatoma接吻虫中检测到的巴尔通体DNA。虽然样本量有限,巴尔通体DNA检测阳性率高，基因序列与人畜共患巴尔通体物种的密切关系，以及人类住宅附近接吻虫子的分布，暗示对公众健康有风险。
■在当前的研究中，在惠州市捕获了22只成年接吻虫（Triatomarubrofasciata），广东省,中国南方。测试了接吻虫子中媒介传播的病原体的存在，并研究了这些潜在病原体的遗传多样性。
■所有的接吻虫子都是无性菌科细菌,立克次体,和柯西拉。在36.4%(8/22)的接吻虫中检测到巴尔通体DNA。巴尔通氏菌gltA基因的序列在系统发育树中分为两个分支，与B.tribocorum和未培养的Bartonellasp。克隆MYR-283。所有groEL序列均与B.kosoyi的序列密切相关（同一性98.75％-100％）。ftsZ和rpoB序列与elizabethae的序列关系最密切，一种公认的人类病原体，核苷酸相似性为98.70%-100%和99.45%-100%，分别。
■我们报告了在中国南方的Triatoma接吻虫中检测到的巴尔通体DNA。虽然样本量有限,巴尔通体DNA检测阳性率高，基因序列与人畜共患巴尔通体物种的密切关系，以及人类住宅附近接吻虫子的分布，暗示对公众健康有风险。
