Abstract：
Genosensor-based electrodes mediated with nanoparticles (NPs) have tremendously developed in medical diagnosis. Herein, we report a facile, rapid, low cost and highly sensitive biosensing strategy for early detection of HPV 18 using gold-nanoparticles (AuNPs) deposited on micro-IDEs. This study represents surface charge transduction of micro-interdigitated electrodes (micro-IDE) alumina insulated with silica, independent and mini genosensor modified with colloidal gold NPs (AuNPs), and determination of gene hybridization for early detection of cervical cancer. The surface of AuNPs deposited micro-IDE functionalized with optimized 3-aminopropyl-triethoxysilane (APTES) followed by hybridization with deoxyribonucleic acid (DNA) virus to develop DNA genosensor. The results of ssDNA hybridization with the ssDNA target of human papillomavirus (HPV) 18 have affirmed that micro-IDE functionalized with colloidal AuNPs resulted in the lowest detection at 0.529 aM. Based on coefficient regression, micro-IDE functionalized with AuNPs produces better results in the sensitivity test (R2 = 0.99793) than unfunctionalized micro-IDE.
摘要：
基于基因传感器的纳米颗粒（NPs）介导的电极在医学诊断中得到了极大的发展。在这里，我们报告一个容易的，快速,低成本和高度敏感的生物传感策略，用于使用沉积在微IDE上的金纳米颗粒（AuNPs）早期检测HPV18。这项研究代表了用二氧化硅绝缘的微叉指形电极（micro-IDE）氧化铝的表面电荷转导，用胶体金NP（AuNP）修饰的独立和微型基因传感器，并确定基因杂交对宫颈癌的早期检测。AuNP的表面沉积了用优化的3-氨基丙基-三乙氧基硅烷（APTES）官能化的微型IDE，然后与脱氧核糖核酸（DNA）病毒杂交以开发DNA基因传感器。ssDNA与人乳头瘤病毒（HPV）18的ssDNA靶标杂交的结果证实，用胶体AuNPs官能化的micro-IDE在0.529aM处的最低检测。根据系数回归，与未功能化的微IDE相比，用AuNP功能化的微IDE在灵敏度测试（R2=0.99793）中产生更好的结果。
