PDF(Pubmed)
Abstract：
The thermal behavior and aggregation process of the poly(N-isopropyl acrylamide), poly[oligo(ethylene glycol) methyl ether methacrylate], and poly[(2-hydroxyethyl methacrylate)-co-oligo(ethylene glycol) methyl ether methacrylate] thermoresponsive polymers were studied in a commonly used Dulbecco\'s Modified Eagle Medium (DMEM) cell culture medium and solutions of its individual components in the same concentration as found in DMEM. All studied copolymers exhibited an unexpected transmittance profile in the DMEM. During heating above the cloud point temperature (TCP), the polymers additionally aggregated, which led to the formation of their precipitates. The behavior of the polymers was further studied to evaluate how individual salts affected the transition temperature, size (Dh), and stability of the polymer particles. Organic additives, such as amino acids and glucose, had a significantly lesser impact on the thermoresponsive aggregation of the polymers than inorganic ones. Changes to the TCP were small and the formation of precipitates was not observed. The presence of small amounts of amino acids caused a decrease in the polymer aggregate sizes. Obtained results are of utmost importance in thermoresponsive drug nanocarrier studies.
摘要：
聚(N-异丙基丙烯酰胺)的热行为和聚集过程,聚[低聚(乙二醇)甲基醚甲基丙烯酸酯]，在常用的Dulbecco的改良Eagle培养基（DMEM）细胞培养基及其各个成分的溶液中研究了聚[（甲基丙烯酸2-羟乙酯）-共聚低聚（乙二醇）甲基醚甲基丙烯酸酯]热响应性聚合物。在DMEM中发现的浓度相同。所有研究的共聚物在DMEM中表现出意想不到的透射率曲线。在高于浊点温度(TCP)的加热过程中,聚合物额外聚集，这导致了它们沉淀的形成。进一步研究了聚合物的行为，以评估单个盐如何影响转变温度，尺寸（Dh），和聚合物颗粒的稳定性。有机添加剂,如氨基酸和葡萄糖，与无机聚合物相比，对聚合物的热响应性聚集的影响明显较小。TCP的变化很小，没有观察到沉淀物的形成。少量氨基酸的存在导致聚合物聚集体尺寸的减小。获得的结果在热响应性药物纳米载体研究中至关重要。
