Abstract：
Brassinin and camalexin belong to phytoalexins, plant compounds generated in the response to stress. Both compounds are known to be cytotoxic to several cancer cell lines, mainly by inducing oxidative stress and subsequent apoptosis. In the presented study, cytotoxic effects of brassinin and camalexin, individually and, for the first time, after combined exposure, on the cells of normal (CCD-Co18) and cancer (Caco-2) lines originated from colorectal tissues and their proapoptotic impact on Caco-2 cells were studied. The determined IC50 values indicate a clearly higher sensitivity of cancer cells to the tested substances, as well as a stronger cytotoxic effect of camalexin than brassinin. The synergistic effect of both phytoalexins was also demonstrated. Caspase-dependent and independent mechanisms were involved in the final effects. Both tested phytoalexins caused evident, concentration-dependent symptoms of oxidative stress in cancer cells, leading to apoptosis, but in the highest concentrations, also to necrosis. In case of camalexin, signs of pyroptosis were additionally detected.
摘要：
Brassinin和camalexin属于植物抗毒素，响应胁迫产生的植物化合物。已知这两种化合物对几种癌细胞系具有细胞毒性，主要通过诱导氧化应激和随后的细胞凋亡。在提出的研究中，Brassinin和camalexin的细胞毒性作用，单独和,第一次,联合暴露后，研究了源自结直肠组织的正常（CCD-Co18）和癌症（Caco-2）细胞系及其对Caco-2细胞的促凋亡作用。确定的IC50值表明癌细胞对测试物质的敏感性明显更高，以及camalexin比brassinin更强的细胞毒性作用。还证明了两种植物抗毒素的协同作用。最终效应涉及胱天蛋白酶依赖和独立的机制。两种测试的植物抗毒素引起了明显的，癌细胞中氧化应激的浓度依赖性症状，导致细胞凋亡，但是在最高浓度下，也是坏死。如果是卡巴解素,此外还检测到焦亡的迹象。
