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Abstract：
Podocyte cellular injury and detachment from glomerular capillaries constitute a critical factor contributing to kidney disease. Notably, transcription factors are instrumental in maintaining podocyte differentiation and homeostasis. This study explores the hitherto uninvestigated expression of Nuclear Factor Erythroid 2-related Factor 1 (NFE2L1) in podocytes. We evaluated the podocyte expression of NFE2L1, Nuclear Factor Erythroid 2-related Factor 2 (NFE2L2), and NAD(P)H:quinone Oxidoreductase (NQO1) in 127 human glomerular disease biopsies using multiplexed immunofluorescence and image analysis. We found that both NFE2L1 and NQO1 expressions were significantly diminished across all observed renal diseases. Furthermore, we exposed human immortalized podocytes and ex vivo kidney slices to Puromycin Aminonucleoside (PAN) and characterized the NFE2L1 protein isoform expression. PAN treatment led to a reduction in the nuclear expression of NFE2L1 in ex vivo kidney slices and podocytes.
摘要：
足细胞损伤和肾小球毛细血管脱离是导致肾脏疾病的关键因素。值得注意的是,转录因子有助于维持足细胞分化和稳态。这项研究探讨了迄今为止尚未研究的核因子红系2相关因子1（NFE2L1）在足细胞中的表达。我们评估了NFE2L1，核因子红系2相关因子2（NFE2L2）的足细胞表达，和NAD（P）H：醌氧化还原酶（NQO1）在127人肾小球疾病活检中使用多重免疫荧光和图像分析。我们发现，在所有观察到的肾脏疾病中，NFE2L1和NQO1的表达均显着减少。此外，我们将人永生化足细胞和离体肾切片暴露于普鲁霉素氨基核苷(PAN),并对NFE2L1蛋白亚型表达进行了表征.PAN处理导致离体肾切片和足细胞中NFE2L1的核表达减少。
