Abstract：
The aim of this study was to evaluate the effect of supplementing bovine semen freezing extender with different concentrations of iodixanol on post-thaw sperm characteristics. Six ejaculates of three Nellore bulls were pooled and diluted in commercial extender (BotuBov®) and then divided into 4 groups: control group (without adding iodixanol); groups G1.5, G3, or G6 according to the concentration of iodixanol solution (RedCushion®). After dilution, the samples were cooled and frozen. Post-thaw semen evaluation included sperm motility by CASA immediately after thawing and after 60 min of incubation at 37°C, flow cytometry analysis for integrity of plasma and acrosomal membranes, membrane destabilization and translocation of phosphatidylserine, mitochondrial membrane potential, and formation of intracellular anion superoxide ( O 2 - ), hydrogen peroxide (H2 O2 ), and membrane lipid peroxidation. The group G6 presented significantly higher (p < .05) total and progressive motility, percentage of plasma and acrosomal membrane integrity, and H2 O2 than control and group G1.5. Furthermore, group G6 showed lower (p < .05) lipid peroxidation than control. In addition, regardless of the concentration used, the percentage of spermatozoa without phosphatidylserine translocation was higher (p < .05) in all iodixanol supplemented groups. In conclusion, iodixanol supplementation preserved the motility and integrity of sperm membranes during cryopreservation and protected against lipid peroxidation.
摘要：
本研究的目的是评估在牛精液冷冻补充剂中添加不同浓度的碘克沙醇对解冻后精子特性的影响。将三个Nellore公牛的六个射精合并并在商业补充剂（BotuBov®）中稀释，然后分为4组：对照组（不添加碘克沙醇）;根据碘克沙醇溶液（RedCushion®）的浓度，组G1.5，G3或G6。稀释后,将样品冷却并冷冻。解冻后精液评估包括解冻后立即通过CASA进行精子运动性，并在37°C下孵育60分钟后，流式细胞术分析血浆和顶体膜的完整性，磷脂酰丝氨酸的膜失稳和易位，线粒体膜电位，和细胞内阴离子超氧化物的形成（O2-${\\\mathrm{O}}_2^{-}$$），过氧化氢(H2O2),和膜脂质过氧化。G6组表现出显着更高（p<0.05）的总运动和进行性运动，血浆和顶体膜完整性的百分比，和H2O2比对照组和G1.5组。此外，G6组显示出比对照组更低的脂质过氧化(p<0.05)。此外,不管使用的浓度，在所有补充碘克沙醇的组中，没有磷脂酰丝氨酸易位的精子百分比更高（p<.05）。总之,补充碘克沙醇可在冷冻保存过程中保留精子膜的运动性和完整性，并防止脂质过氧化。
