PDF(Pubmed)
Abstract：
Since the dawn of civilization, people have turned to plants as a safe and efficient form of treatment for a variety of diseases. It has long been known that Calotropis procera has the potential to treat a number of diseases. In this study, the C. procera leaf aqueous extract was obtained using the maceration method, and p-coumaric was found to be the main compound. The extract was rich in phenols (174.82 mg gallic acid equivalent/g) and flavonoids (1781.7 µg quercetin equivalent/g). The extract had high antioxidant properties, as indicated by the IC50 values obtained for 2,2-diphenyl-1-picrylhydrazyl (DPPH) (366.33 μg/mL) and 2,2\'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) (169.04 μg/mL), as well as the ferric ions reducing antioxidant power (FRAP) (1.67 μg ascorbic acid equivalent/g of the extract). The cytotoxicity of the extract was evaluated against the survival of HT 29 cells, and the IC50 was found to be 236.87 μg/mL. The most resistant and sensitive strains to the extract were Escherichia coli and Staphylococcus aureus, respectively. The morphological changes of these strains were demonstrated through scanning electron microscopy and confocal laser scanning microscopy. The C. procera extract could be therefore used as an antioxidant, antimicrobial, and anticancer agent.
摘要：
从文明的黎明开始，人们已经把植物作为一种安全有效的治疗各种疾病的方法。早就知道Calotropisprocera具有治疗多种疾病的潜力。在这项研究中,C.procera叶水提取物是使用浸渍法获得的，发现p-香豆酸是主要化合物。提取物富含酚类（174.82mg没食子酸当量/g）和类黄酮（1781.7µg槲皮素当量/g）。提取物具有很高的抗氧化性能，如2,2-二苯基-1-吡啶酰肼(DPPH)(366.33μg/mL)和2,2'-氮杂-双(3-乙基苯并噻唑啉-6-磺酸(ABTS)(169.04μg/mL)获得的IC50值所示，以及铁离子还原抗氧化能力（FRAP）（1.67μg抗坏血酸当量/g提取物）。针对HT29细胞的存活评估提取物的细胞毒性，IC50为236.87μg/mL。对提取物耐药和敏感程度最高的菌株为大肠埃希菌和金黄色葡萄球菌,分别。通过扫描电子显微镜和共聚焦激光扫描显微镜证明了这些菌株的形态变化。因此，C.procera提取物可以用作抗氧化剂，抗菌，和抗癌剂。
