Abstract：
This study investigated the multiproduct (fucoidans, β-glucans, proteins, carotenoids, fatty acids, amino acids and polyphenols) valorization of the invasive macroalgae Ascophyllum nodosum within a green biorefinery concept using ultrasound (US), microwave (MW) treatment followed by supercritical CO2 (SC-CO2) with co-solvent and enzymatic extraction. Water and 50% aqueous ethanol were used as green extraction solvents. The extraction methods using 50% ethanol as extraction solvent improved the yields of phenolic compounds and glucan and enhanced in vitro antioxidant activity. The characterization of SC-CO2 extracts revealed that pretreatment with US and MW improved the 2-fold yield of carotenoids, total phenolics and fatty acids. However, US/MW pretreatment and enzymatic extraction did not improve the yields of proteins and free amino acids. Overall, using concurrent green US/MW-assisted extraction methods enhanced the yields of the bioactive compounds in a short duration and provided extracts with a better antioxidant capacity in the field of food applications.
摘要：
这项研究调查了多产品(岩藻依聚糖，β-葡聚糖，蛋白质，类胡萝卜素，脂肪酸，氨基酸和多酚）使用超声波（US）在绿色生物炼制概念中对侵袭性大型藻类夜蛾进行增值，微波（MW）处理，然后用超临界CO2（SC-CO2）与共溶剂和酶法提取。水和50%含水乙醇用作绿色提取溶剂。使用50％乙醇作为提取溶剂的提取方法提高了酚类化合物和葡聚糖的产率，并增强了体外抗氧化活性。SC-CO2提取物的表征表明，用US和MW预处理可提高类胡萝卜素的2倍产量，总酚和脂肪酸。然而,US/MW预处理和酶提取不能提高蛋白质和游离氨基酸的产量。总的来说,同时使用绿色US/MW辅助提取方法可在短时间内提高生物活性化合物的产量，并在食品应用领域提供具有更好抗氧化能力的提取物。
