PDF(Pubmed)
Abstract：
OBJECTIVE: The main aim/objective of this study was to detect and characterize the Brucella species from patients having complaints of joint pain and also to know the potential causes of human brucellosis. In our study, we focused on joint pain symptoms that may be due to arthralgia or arthritis. Introduction: Brucellosis is a neglected zoonotic disease that affects both humans and animals. In humans, brucellosis begins with chronic illness leading to great financial losses from not being able to work well and continued treatment costs, but few such studies have come from northern India. Joint pain is the common presentation of brucellosis and there are several risk factors associated with brucellosis.
METHODS: A total of 200 blood samples were collected from the participants having joints pain from September 2019 to September 2021 at Gandhi Memorial & Associated Hospitals of King George\'s Medical University, Lucknow, India, and tested by serology for anti-Brucella IgM and IgG enzyme-linked immunosorbent assay (ELISA), molecular tests byreverse transcriptase-polymerase chain reaction (RT-PCR), conventional polymerase chain reaction (PCR), and automated blood culture system. The anti-Brucella IgM and IgG ELISA were performed using the kit from NovaTec Immundiagnostica GmbH (Dietzenbach, Germany). Isolation of DNA was carried out using the QIAamp DNA Mini kit (QIAGEN, Hilden, Germany), and the primers and probes specific for targeted regions (BCSP31 and IS711 gene) in the Brucella genome were procured from Eurofins Scientific SE (Luxembourg, France), and for internal control from CDC.
RESULTS: The study showed 19 (9.5%) and 23 (11.5%) positive results by anti-Brucella IgM ELISA and anti-Brucella IgG, respectively, and of these, one (0.5%) was positive for both anti-Brucella IgM and anti-Brucella IgG ELISA. Out of 19 anti-Brucella IgM ELISA positive, eight (4%) samples were positive for PCR/RT-PCR and that was negative for anti-Brucella IgG ELISA. All blood culture reports of all patients were negative. Conclusion: Anti-Brucella IgM ELISA was more accurate than anti-Brucella IgG ELISA in detecting human brucellosis. Consumption of animal products (i.e. milk, a dairy product of cow, buffalo, goat, and meat of goat) and contact with animals were the main risk factors that were identified for Brucella disease.
METHODS: A total of 200 blood samples were collected from the participants having joints pain from September 2019 to September 2021 at Gandhi Memorial & Associated Hospitals of King George\'s Medical University, Lucknow, India, and tested by serology for anti-Brucella IgM and IgG enzyme-linked immunosorbent assay (ELISA), molecular tests byreverse transcriptase-polymerase chain reaction (RT-PCR), conventional polymerase chain reaction (PCR), and automated blood culture system. The anti-Brucella IgM and IgG ELISA were performed using the kit from NovaTec Immundiagnostica GmbH (Dietzenbach, Germany). Isolation of DNA was carried out using the QIAamp DNA Mini kit (QIAGEN, Hilden, Germany), and the primers and probes specific for targeted regions (BCSP31 and IS711 gene) in the Brucella genome were procured from Eurofins Scientific SE (Luxembourg, France), and for internal control from CDC.
RESULTS: The study showed 19 (9.5%) and 23 (11.5%) positive results by anti-Brucella IgM ELISA and anti-Brucella IgG, respectively, and of these, one (0.5%) was positive for both anti-Brucella IgM and anti-Brucella IgG ELISA. Out of 19 anti-Brucella IgM ELISA positive, eight (4%) samples were positive for PCR/RT-PCR and that was negative for anti-Brucella IgG ELISA. All blood culture reports of all patients were negative. Conclusion: Anti-Brucella IgM ELISA was more accurate than anti-Brucella IgG ELISA in detecting human brucellosis. Consumption of animal products (i.e. milk, a dairy product of cow, buffalo, goat, and meat of goat) and contact with animals were the main risk factors that were identified for Brucella disease.
摘要：
目的：这项研究的主要目的/目的是检测和鉴定患有关节痛的患者的布鲁氏菌种类，并了解人类布鲁氏菌病的潜在原因。在我们的研究中,我们专注于关节疼痛症状，可能是由于关节痛或关节炎。简介：布鲁氏菌病是一种被忽视的人畜共患疾病，影响人类和动物。在人类中,布鲁氏菌病始于慢性疾病，导致无法正常工作和持续治疗费用带来巨大的经济损失，但是很少有这样的研究来自印度北部。关节疼痛是布鲁氏菌病的常见表现，并且有几种与布鲁氏菌病相关的危险因素。
方法：从2019年9月至2021年9月在乔治国王医科大学的甘地纪念和联合医院收集了200份来自关节疼痛的参与者的血液样本。勒克瑙,印度,并通过血清学检测抗布鲁氏菌IgM和IgG酶联免疫吸附试验(ELISA),通过逆转录酶-聚合酶链反应（RT-PCR）进行的分子测试，常规聚合酶链反应（PCR），和自动化血液培养系统。使用NovaTecImmundiagnosticaGmbH（Dietzenbach，德国)。使用QIAampDNAMini试剂盒（QIAGEN，希尔登,德国),布鲁氏菌基因组中针对目标区域（BCSP31和IS711基因）的引物和探针是从EurofinsScientificSE（卢森堡，法国),以及CDC的内部控制。
结果：通过抗布鲁氏菌IgMELISA和抗布鲁氏菌IgG，该研究显示19（9.5％）和23（11.5％）阳性结果，分别,其中，一个（0.5％）的抗布鲁氏菌IgM和抗布鲁氏菌IgGELISA均为阳性。19种抗布鲁氏菌IgMELISA阳性,8份（4％）样品的PCR/RT-PCR阳性，抗布氏杆菌IgGELISA阴性。所有患者的血培养报告均为阴性。结论:抗布鲁氏菌IgMELISA比抗布鲁氏菌IgGELISA更准确地检测人布鲁氏菌病。消费动物产品(即牛奶，奶牛的乳制品，水牛,山羊,和山羊肉）和与动物的接触是布鲁氏菌病的主要危险因素。
方法：从2019年9月至2021年9月在乔治国王医科大学的甘地纪念和联合医院收集了200份来自关节疼痛的参与者的血液样本。勒克瑙,印度,并通过血清学检测抗布鲁氏菌IgM和IgG酶联免疫吸附试验(ELISA),通过逆转录酶-聚合酶链反应（RT-PCR）进行的分子测试，常规聚合酶链反应（PCR），和自动化血液培养系统。使用NovaTecImmundiagnosticaGmbH（Dietzenbach，德国)。使用QIAampDNAMini试剂盒（QIAGEN，希尔登,德国),布鲁氏菌基因组中针对目标区域（BCSP31和IS711基因）的引物和探针是从EurofinsScientificSE（卢森堡，法国),以及CDC的内部控制。
结果：通过抗布鲁氏菌IgMELISA和抗布鲁氏菌IgG，该研究显示19（9.5％）和23（11.5％）阳性结果，分别,其中，一个（0.5％）的抗布鲁氏菌IgM和抗布鲁氏菌IgGELISA均为阳性。19种抗布鲁氏菌IgMELISA阳性,8份（4％）样品的PCR/RT-PCR阳性，抗布氏杆菌IgGELISA阴性。所有患者的血培养报告均为阴性。结论:抗布鲁氏菌IgMELISA比抗布鲁氏菌IgGELISA更准确地检测人布鲁氏菌病。消费动物产品(即牛奶，奶牛的乳制品，水牛,山羊,和山羊肉）和与动物的接触是布鲁氏菌病的主要危险因素。
