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Abstract：
Fluorescent dyes are used in biological systems, because they are highly sensitive and selective. In this work, we investigated the fluorescent probe properties of 2-(5-(pyridin-2-yl)-1H-pyrazol-3-yl) phenol (PYDP) in two media [sodium dodecyl sulfate (SDS) and human serum albumin (HSA)]. Energy transfer parameters, photophysical and thermodynamic parameters of probe were determined. We investigated cytotoxicity of PYDP against colorectal adenocarcinoma cell lines (HT-29), breast cancer cell lines (MCF-7) and 3T3-L1 adipocytes (3T3 L1) cells. The cell staining property of PYDP was monitored using a confocal microscope. The results showed that PYDP binds to HSA, bindings are due to electrostatic/ionic interactions, and the binding process is spontaneous. PYDP was found to exhibit negligible cytotoxicity at high concentrations, and confocal microscope images showed that PYDP stained the cytoplasm of MCF-7 cells.
摘要：
荧光染料用于生物系统，因为它们是高度敏感和选择性的。在这项工作中,我们研究了2-（5-（吡啶-2-基）-1H-吡唑-3-基）苯酚（PYDP）在两种介质[十二烷基硫酸钠（SDS）和人血清白蛋白（HSA）]中的荧光探针特性。能量转移参数,确定了探针的光物理和热力学参数。我们研究了PYDP对结直肠腺癌细胞系（HT-29）的细胞毒性，乳腺癌细胞系（MCF-7）和3T3-L1脂肪细胞（3T3L1）细胞。使用共聚焦显微镜监测PYDP的细胞染色性质。结果显示PYDP与HSA结合,结合是由于静电/离子相互作用，结合过程是自发的。发现PYDP在高浓度下表现出可忽略的细胞毒性，共聚焦显微镜图像显示PYDP染色MCF-7细胞的细胞质。
